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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Detection of polymorphisms among theileria parva stocks using repetitive, telomeric and ribosomal dna probes and anti-schizont monoclonal antibodies
Parasitology, Volume 107, No. 1, Year 1993
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Description
A total of 21 Theileria parva stocks from 6 countries were characterized using T. parva repetitive and ribosomal DNA probes, a Plasmodium berghei telomeric oligonucleotide and a panel of anti-schizont monoclonal antibodies (MAbs). Hybridization of the repetitive DNA probe to Southern blots of EcoRI-digested T. parva DNA revealed 20 different restriction fragment patterns among DNA samples isolated from infections initiated using 16 parasite stocks. The panel of anti-schizont MAbs defined 8 different profiles among schizont-infected lymphoblastoid cell-cultures infected with the same 16 T. parva stocks. Many stocks, which were differentiated by the repetitive DNA probe, could not be distinguished using the anti-schizont MAbs. A cloned T. parva small subunit ribosomal RNA (SSUrRNA) gene probe separated 17 T. parva stocks into 2 groups, exhibiting either 1 or 2 restriction fragments, when hybridized to EcoRI-digested T. parva DNA. When hybridized to PvuII-digested DNA from 8 T. parva stocks, the ribosomal probe identified 4 groups with similar restriction fragment patterns. A synthetic oligonucleotide derived from a P. berghei telomeric sequence hybridized to 7 or 8 size-polymorphic restriction fragments in the EcoRI-digested DNA of most T. parva stocks. The telomeric and ribosomal probes defined the same 4 groups among 8 T. parva stocks as assessed by similarities in restriction fragment patterns. Based on the comparison of repetitive DNA sequences from the T. parva Uganda and Muguga stocks, a synthetic oligonucleotide was developed which distinguished the DNA of the T. parva Uganda stock from that of 4 other T. parva stocks on a positive/negative basis. © 1993, Cambridge University Press. All rights reserved.
Authors & Co-Authors
Bishop, Richard Peter
Unknown Affiliation
Sohanpal, Baljinder K.
Unknown Affiliation
Allsopp, V. B.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Spooner, Paul R.
Unknown Affiliation
Dolan, Thomas T.
Unknown Affiliation
Morzaria, Subhash P.
Unknown Affiliation
Statistics
Citations: 53
Authors: 6
Affiliations: 1
Identifiers
Doi:
10.1017/S0031182000079361
e-ISSN:
14698161
Research Areas
Genetics And Genomics
Study Locations
Uganda