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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Assessment of the genetic diversity of the Tunisian citrus rootstock germplasm
BMC Genetics, Volume 13, Article 16, Year 2012
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Description
Background: Citrus represents a substantial income for farmers in the Mediterranean Basin. However, the Mediterranean citrus industry faces increasing biotic and abiotic constraints. Therefore the breeding and selection of new rootstocks are now of the utmost importance. In Tunisia, in addition to sour orange, the most widespread traditional rootstock of the Mediterranean area, other citrus rootstocks and well adapted to local environmental conditions, are traditionally used and should be important genetic resources for breeding. To characterize the diversity of Tunisian citrus rootstocks, two hundred and one local accessions belonging to four facultative apomictic species (C. aurantium, sour orange; C. sinensis, orange; C. limon, lemon; and C. aurantifolia, lime) were collected and genotyped using 20 nuclear SSR markers and four indel mitochondrial markers. Multi-locus genotypes (MLGs) were compared to references from French and Spanish collections.Results: The differentiation of the four varietal groups was well-marked. The groups displayed a relatively high allelic diversity, primarily due to very high heterozygosity. Sixteen distinct MLGs were identified. Ten of these were noted in sour oranges. However, the majority of the analysed sour orange accessions corresponded with only two MLGs, differentiated by a single allele, likely due to a mutation. The most frequent MLG is shared with the reference sour oranges. No polymorphism was found within the sweet orange group. Two MLGs, differentiated by a single locus, were noted in lemon. The predominant MLG was shared with the reference lemons. Limes were represented by three genotypes. Two corresponded to the 'Mexican lime' and 'limonette de Marrakech' references. The MLG of 'Chiiri' lime was unique.Conclusions: The Tunisian citrus rootstock genetic diversity is predominantly due to high heterozygosity and differentiation between the four varietal groups. The phenotypic diversity within the varietal groups has resulted from multiple introductions, somatic mutations and rare sexual recombination events. Finally, this diversity study enabled the identification of a core sample of accessions for further physiological and agronomical evaluations. These core accessions will be integrated into citrus rootstock breeding programs for the Mediterranean Basin. © 2012 Snoussi et al; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3323426/bin/1471-2156-13-16-S1.PDF
Authors & Co-Authors
Snoussi, Hager
Tunisia, Tunis
University of Carthage, Institut National de la Recherche Agronomique de Tunisie
Duval, M. F.
France, Paris
Cirad
García-Lor, Andrés
Spain, Moncada
Instituto Valenciano de Investigaciones Agrarias
Belfalah, Zina
Tunisia, Tunis
University of Carthage, Institut National de la Recherche Agronomique de Tunisie
Froelicher, Yann
France, Paris
Cirad
Risterucci, Ange Marie
France, Paris
Cirad
Perrier, Xavier
France, Paris
Cirad
Jacquemoud-Collet, Jean Pierre
France, Paris
Cirad
Navarro, Luís
Spain, Moncada
Instituto Valenciano de Investigaciones Agrarias
Harrabi, Moncef
Tunisia, Tunis
University of Carthage, Institut National Agronomique de Tunisie
Ollitrault, Patrick
France, Paris
Cirad
Spain, Moncada
Instituto Valenciano de Investigaciones Agrarias
Statistics
Citations: 29
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.1186/1471-2156-13-16
e-ISSN:
14712156
Research Areas
Cancer
Genetics And Genomics
Sexual And Reproductive Health
Study Locations
Tunisia