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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses
Nature Communications, Volume 12, No. 1, Article 2055, Year 2021
Notification
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Description
Identification of protective T cell responses against SARS-CoV-2 requires distinguishing people infected with SARS-CoV-2 from those with cross-reactive immunity to other coronaviruses. Here we show a range of T cell assays that differentially capture immune function to characterise SARS-CoV-2 responses. Strong ex vivo ELISpot and proliferation responses to multiple antigens (including M, NP and ORF3) are found in 168 PCR-confirmed SARS-CoV-2 infected volunteers, but are rare in 119 uninfected volunteers. Highly exposed seronegative healthcare workers with recent COVID-19-compatible illness show T cell response patterns characteristic of infection. By contrast, >90% of convalescent or unexposed people show proliferation and cellular lactate responses to spike subunits S1/S2, indicating pre-existing cross-reactive T cell populations. The detection of T cell responses to SARS-CoV-2 is therefore critically dependent on assay and antigen selection. Memory responses to specific non-spike proteins provide a method to distinguish recent infection from pre-existing immunity in exposed populations. © 2021, The Author(s).
Authors & Co-Authors
Ogbe, Ane T.
United Kingdom, Oxford
University of Oxford
Skelly, Dónal Thomas
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
Pace, Matthew J.
United Kingdom, Oxford
University of Oxford
Brown, Anthony
United Kingdom, Oxford
University of Oxford
Adland, Emily
United Kingdom, Oxford
University of Oxford
Ali, Mohammad I.
United Kingdom, Oxford
University of Oxford
Ansari, M. Azim
United Kingdom, Oxford
University of Oxford
Brown, Helen L.
United Kingdom, Oxford
University of Oxford
Chinnakannan, Senthil K.
United Kingdom, Oxford
University of Oxford
Conlon, Christopher P.
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
de Lara, Catherine M.
United Kingdom, Oxford
University of Oxford
de Silva, Thushan I.
United Kingdom, Sheffield
The University of Sheffield
Dold, Christina
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Nihr Oxford Biomedical Research Centre
Dong, Tao
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Donnison, Timothy A.
United Kingdom, Oxford
University of Oxford
Eyre, David William
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
United Kingdom, Oxford
University of Oxford Medical Sciences Division
Flaxman, Amy L.
United Kingdom, Oxford
The Jenner Institute
Fletcher, Helen A.
United Kingdom, London
London School of Hygiene & Tropical Medicine
Hackstein, Carl Philipp
United Kingdom, Oxford
University of Oxford
Jeffery, Katie J.M.
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
Lambe, Teresa
United Kingdom, Oxford
The Jenner Institute
Lee, Lianni
United Kingdom, Oxford
University of Oxford
Li, Wenqin
United Kingdom, Oxford
University of Oxford
Lim, Nicholas
United Kingdom, Oxford
University of Oxford
Matthews, Philippa C.
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
Mentzer, Alexander J.
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
United Kingdom, Oxford
University of Oxford
Moore, Shona C.
United Kingdom, Liverpool
University of Liverpool
Naisbitt, Dean John
United Kingdom, Liverpool
University of Liverpool
Ogg, Graham Stuart
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
United Kingdom, Oxford
Nihr Oxford Biomedical Research Centre
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Openshaw, Peter J.M.
United Kingdom, London
Imperial College Faculty of Medicine
Pirmohamed, Munir
United Kingdom, Liverpool
University of Liverpool
Pollard, Andrew J.
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Nihr Oxford Biomedical Research Centre
Ramamurthy, Narayan
United Kingdom, Oxford
University of Oxford
Rongkard, Patpong
United Kingdom, Oxford
University of Oxford
Thailand, Bangkok
Mahidol Oxford Tropical Medicine Research Unit
Rowland-Jones, Sarah Louise
United Kingdom, Sheffield
The University of Sheffield
United Kingdom, Oxford
University of Oxford
Sampson, Oliver L.
United Kingdom, Oxford
University of Oxford
Screaton, Gavin Robert
United Kingdom, Oxford
University of Oxford
Sette, Alessandro D.
United States, San Diego
La Jolla Institute for Immunology
United States, Los Angeles
University of California, Los Angeles
Stafford, Lizzie
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
Thompson, Craig Peter
United Kingdom, Oxford
University of Oxford
Thwaites, Ryan S.
United Kingdom, London
Imperial College Faculty of Medicine
Vieira, Vinicius A.
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
University of Oxford Medical Sciences Division
Weiskopf, Daniela
United States, San Diego
La Jolla Institute for Immunology
United States, Los Angeles
University of California, Los Angeles
Zacharopoulou, Panagiota
United Kingdom, Oxford
University of Oxford
Chalk, Jeremy
United Kingdom, Oxford
University of Oxford
Phalora, Prabhjeet K.
United Kingdom, Oxford
University of Oxford
Csala, Anna
United Kingdom, Oxford
University of Oxford
Jones, Matthew G.
United Kingdom, Oxford
University of Oxford
Robinson, Nicola
United Kingdom, Oxford
University of Oxford
Hutchings, Claire L.
United Kingdom, Oxford
University of Oxford
Amini, Ali
United Kingdom, Oxford
University of Oxford
Dimitriadis, Stavros
United Kingdom, Oxford
University of Oxford
Johnson, Síle Ann
United Kingdom, Oxford
University of Oxford
Turtle, Lance C.W.
United Kingdom, Liverpool
University of Liverpool
United Kingdom, Liverpool
Liverpool Health Partners
Klenerman, Paul
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
United Kingdom, Oxford
Nihr Oxford Biomedical Research Centre
Goulder, Philip Jeremy Renshaw
United Kingdom, Oxford
University of Oxford Medical Sciences Division
Frater, John
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
United Kingdom, Oxford
Nihr Oxford Biomedical Research Centre
Barnes, Eleanor J.
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
United Kingdom, Oxford
Nihr Oxford Biomedical Research Centre
Dunachie, Susanna J.
United Kingdom, Oxford
University of Oxford
United Kingdom, Oxford
Oxford University Hospitals Nhs Foundation Trust
United Kingdom, Oxford
Nihr Oxford Biomedical Research Centre
Thailand, Bangkok
Mahidol Oxford Tropical Medicine Research Unit
Statistics
Citations: 75
Authors: 59
Affiliations: 15
Identifiers
Doi:
10.1038/s41467-021-21856-3
ISSN:
20411723
Research Areas
Covid
Health System And Policy