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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Dietary red palm oil supplementation reduces myocardial infarct size in an isolated perfused rat heart model
Lipids in Health and Disease, Volume 9, Article 64, Year 2010
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Description
Background and Aims: Recent studies have shown that dietary red palm oil (RPO) supplementation improves functional recovery following ischaemia/reperfusion in isolated hearts. The main aim of this study was to investigate the effects of dietary RPO supplementation on myocardial infarct size after ischaemia/reperfusion injury. The effects of dietary RPO supplementation on matrix metalloproteinase-2 (MMP2) activation and PKB/Akt phosphorylation were also investigated. Materials and methods. Male Wistar rats were divided into three groups and fed a standard rat chow diet (SRC), a SRC supplemented with RPO, or a SRC supplemented with sunflower oil (SFO), for a five week period, respectively. After the feeding period, hearts were excised and perfused on a Langendorff perfusion apparatus. Hearts were subjected to thirty minutes of normothermic global ischaemia and two hours of reperfusion. Infarct size was determined by triphenyltetrazolium chloride staining. Coronary effluent was collected for the first ten minutes of reperfusion in order to measure MMP2 activity by gelatin zymography. Results: Dietary RPO-supplementation decreased myocardial infarct size significantly when compared to the SRC-group and the SFO-supplemented group (9.1 1.0% versus 30.2 3.9% and 27.1 2.4% respectively). Both dietary RPO- and SFO-supplementation were able to decrease MMP2 activity when compared to the SRC fed group. PKB/Akt phosphorylation (Thr 308) was found to be significantly higher in the dietary RPO supplemented group when compared to the SFO supplemented group at 10 minutes into reperfusion. There was, however, no significant changes observed in ERK phosphorylation. Conclusions: Dietary RPO-supplementation was found to be more effective than SFO-supplementation in reducing myocardial infarct size after ischaemia/reperfusion injury. Both dietary RPO and SFO were able to reduce MMP2 activity, which suggests that MMP2 activity does not play a major role in protection offered by RPO. PKB/Akt phosphorylation may, however, be involved in RPO mediated protection. © 2010 Bester et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Bester, Dirk J.
South Africa, Bellville
Cape Peninsula University of Technology
Kupai, Krisztina
Hungary, Szeged
Cardiovascular Research Group, Szeged
Csont, Tamás
Hungary, Szeged
Cardiovascular Research Group, Szeged
Hungary, Budapest
Pharmahungary Group
Szücs, Gergö
Hungary, Szeged
Cardiovascular Research Group, Szeged
Csonka, Csaba
Hungary, Szeged
Cardiovascular Research Group, Szeged
Hungary, Budapest
Pharmahungary Group
Esterhuyse, Adriaan J.
South Africa, Bellville
Cape Peninsula University of Technology
Ferdinandy, Peter
Hungary, Szeged
Cardiovascular Research Group, Szeged
Hungary, Budapest
Pharmahungary Group
van Rooyen, Jacques
South Africa, Bellville
Cape Peninsula University of Technology
Statistics
Citations: 42
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1186/1476-511X-9-64
e-ISSN:
1476511X
Research Areas
Violence And Injury
Participants Gender
Male