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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Three genes and two isozymes: gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense
Molecular and Biochemical Parasitology, Volume 69, No. 2, Year 1995
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Description
The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of Trypanosoma (Nannomonas) congolense. The organization of the genes within the complex is similar to that of Trypanosoma brucei brucei. The nucleotide and amino-acid sequences, including those of the novel high-molecular-weight 56PGK, show substantial cross-species similarity. However, the two downstream genes, c1PGK and c2PGK, encode identical isozymes in T. congolense, while they encode distinct glycosomal and cytoplasmic isozymes in T. brucei. Western analysis also indicated that there are only two isozymes in T. congolense and that these are constitutively expressed. Differential digitonin solubilization of the trypanosomes indicated that 56PGK is primarily localized to the glycosome, as expected, and that c1 c2PGK is cytoplasmic. Northern analysis demonstrates that while 56PGK is constitutively expressed, c1PGK and c2PGK mRNAs are differentially expressed in the T. congolense developmental stages. This work demonstrates that T. congolense has only one PGK isozyme, 56PGK, that is predominantly localized in glycosomes. © 1995.
Authors & Co-Authors
Parker, Heather L.
United States, Seattle
Seattle Biomedical Research Institute
Hill, Teresa
United States, Seattle
Seattle Biomedical Research Institute
Alexander, Keith
United States, Seattle
Seattle Biomedical Research Institute
Murphy, Noel B.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Fish, Wallace R.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Parsons, Marilyn
United States, Seattle
Seattle Biomedical Research Institute
United States, Seattle
University of Washington
Statistics
Citations: 55
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/0166-6851(94)00208-5
ISSN:
01666851
Research Areas
Genetics And Genomics