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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains
Journal of Clinical Virology, Volume 48, No. 3, Year 2010
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Description
Background: Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations. Objectives: To develop a real-time assay for YFV able to detect African and South American strains. Study design: Three short probe (14-18 nt) formats were compared and a plasmid-transcribed RNA standard was used to test the performance of the assays. Additionally the new TaqM1 enzyme was tested. Results: A locked nucleotide probe (LNA probe) performed best with an analytical sensitivity of 10 RNA molecules detected. 44 African and 10 South American strains were detectable. One South American strain from 1984 had a one-nucleotide deviation in the hybridisation sequence for which the LNA probe had to be adapted. Comparison of enzymes revealed that not all enzymes are suitable for LNA probes. Conclusion: The developed LNA probe based YFV real-time PCR performed best in an enzyme mix and less efficient using multifunctional enzymes. © 2010 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Weidmann, Manfred W.
Germany, Gottingen
Universitätsmedizin Göttingen
Faye, Ousmane C.
Senegal, Dakar
Institut Pasteur de Dakar
Faye, Oumar K.
Senegal, Dakar
Institut Pasteur de Dakar
Kranaster, Ramon
Germany, Konstanz
Universität Konstanz
Marx, Andreas
Germany, Konstanz
Universität Konstanz
Nunes, Marcio R.T.
Brazil, Ananindeua
Instituto Evandro Chagas
Vasconcelos, Pedro V.
Brazil, Ananindeua
Instituto Evandro Chagas
Hufert, Frank T.
Germany, Gottingen
Universitätsmedizin Göttingen
Sall, Amadou Alpha
Senegal, Dakar
Institut Pasteur de Dakar
Statistics
Citations: 55
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1016/j.jcv.2010.04.013
ISSN:
13866532
Research Areas
Infectious Diseases