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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Viral load in tissues during the early and chronic phase of non-pathogenic SIVagm infection
Journal of Medical Primatology, Volume 33, No. 2, Year 2004
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Description
African green monkeys (AGMs) persistently infected with SIVagm do not develop AIDS, although their plasma viremia levels can reach those reported for pathogenic HIV-1 and SIVmac infections. In contrast, the viral burden in lymph nodes in SIVagm-infected AGMs is generally lower in comparison with HIV/SIVmac pathogenic infections, at least during the chronic phase of SIVagm infection. We searched for the primary targets of viral replication, which might account for the high viremias in SIVagm-infected AGMs. We evaluated for the first time during primary infection SIVagm dissemination in various lymphoid and non-lymphoid tissues. Sixteen distinct organs at a time point corresponding to maximal virus production were analyzed for viral RNA and DNA load. At days 8 and 9 p.i., viral RNA could be detected in a wide range of tissues, such as jejunum, spleen, mesenteric lymph nodes, thymus and lung. Quantification of viral DNA and RNA as well as of productively infected cells revealed that viral replication during this early phase takes place mainly in secondary lymphoid organs and in the gut (5 × 104-5 × 108 RNA copies/106 cells). By 4 years p.i., RNA copy numbers were below detection level in thymus and lung. Secondary lymphoid organs displayed 6 × 102-2 × 106 RNA copies/106 cells, while some tissue fragments of ileum and jejunum still showed high viral loads (up to 10 9 copies/106 cells). Altogether, these results indicate a rapid dissemination of SIVagm into lymphoid tissues, including the small intestine. The latter, despite showing marked regional variations, most likely contributes significantly to the high levels of viremia observed during SIVagm infection. © Blackwell Munksgaard, 2004.
Authors & Co-Authors
Gueye, A.
France, Paris
Institut Pasteur, Paris
Diop, Ousmane Madiagne
Senegal, Dakar
Institut Pasteur de Dakar
Ploquin, Mickaël J.Y.
France, Paris
Institut Pasteur, Paris
Kornfeld, Christopher
France, Paris
Institut Pasteur, Paris
Cumont, Marie Christine
France, Paris
Institut Pasteur, Paris
Faye, Abdourahmane
France, Paris
Institut Pasteur, Paris
Hurtrel, Bruno
France, Paris
Institut Pasteur, Paris
Barré-Sinoussi, Françoise C.
France, Paris
Institut Pasteur, Paris
Müller-Trutwin, Michaela C.
France, Paris
Institut Pasteur, Paris
Statistics
Citations: 59
Authors: 9
Affiliations: 2
Identifiers
Doi:
10.1111/j.1600-0684.2004.00057.x
ISSN:
00472565
Research Areas
Genetics And Genomics
Infectious Diseases