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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
environmental science
Decolourization and detoxification of textile industry wastewater by the laccase-mediator system
Journal of Hazardous Materials, Volume 175, No. 1-3, Year 2010
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Description
Decolourization and detoxification of a textile industry effluent by laccase from Trametes trogii in the presence and the absence of laccase mediators was investigated. Laccase alone was not able to decolourize the effluent efficiently even at the highest enzyme concentration tested: less than 10% decolourization was obtained with 9 U/mL reaction mixture. To enhance effluent decolourization, several potential laccase mediators were tested at concentrations ranging from 0 to 1 mM. Most potential mediators enhanced decolourization of the effluent, with 1-hydroxybenzotriazol (HBT) being the most effective. The effect of several physico-chemical parameters that could influence enzyme activity, such as pH, temperature and dye concentration was tested. Optimal decolourization occurred with 20% effluent at pH 5, a temperature of 50 °C, and in the presence of 1 mM HBT. The toxicities of crude, laccase-HBT treated and laccase-acetosyringone treated effluent were evaluated using the Microtox assay. Only laccase-acetosyringone treated effluent was not toxic; crude and laccase-HBT treated effluent retained toxicity. © 2009 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Khlifi, Rim
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Belbahri, L.
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Woodward, Steve
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Ellouz, Mariem
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Dhouib, Abdelhafidh
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Sayadi, Sami
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Mechichi, Tahar
Tunisia, Sfax
Centre de Biotechnologie de Sfax
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Statistics
Citations: 197
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.jhazmat.2009.10.079
ISSN:
03043894
Research Areas
Environmental