4-oxonicotinamide-1-(1'-β-D-ribofuranoside) from Rothmannia longiflora Salisb. (Rubiaceae)

The first isolation of 4-oxonicotinamide-1-(1'-β-D-ribofuranoside) from a nonmammalian source, the West African plant Rothmannia longiflora Salisb. (Rubiaceae), is described. Its stereostructure was established by spectroscopic methods and an X-ray structure analysis of its tri-O-acetyl derivative. This N-glycoside had previously been isolated only from human urine. In addition, the plant was found to contain monomethyl fumarate and D- mannitol.