Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Molecular characterisation of Mycobacterium bovis isolated from African buffaloes (Syncerus caffer) in Hluhluwe-iMfolozi Park in KwaZulu-Natal, South Africa
Onderstepoort Journal of Veterinary Research, Volume 78, No. 1, Year 2011
Notification
URL copied to clipboard!
Description
Bovine tuberculosis (BTB), a chronic disease of mammals caused by Mycobacterium bovis, is a threat to South African wildlife. It has been reported that African buffaloes (Syncerus caffer) are reservoir hosts of BTB in South African wildlife populations. This study reports on the molecular identification and typing of 31 M. bovisisolates collected between 1993 and 2008, mainly from buffaloes but also from two lions and a bush pig, in the Hluhluwe-iMfolozi Park (HiP) in KwaZulu-Natal. To study the dynamics of BTB in the buffalo populations, 28M. bovis isolates from the HiP and epidemiologically related parks were characterised using regions of difference deletion analysis for species identification and spoligotyping, variable number of tandem repeats (VNTR), polymorphic G-C-rich sequences and IS6110restriction fragment length polymorphism (RFLP) genotyping methods. At least three distinct M. bovisgenotypes were found amongst HiP samples. The combination of VNTR typing (using a 16-loci panel) and IS6110RFLP revealed the presence of three additional genetic profiles in individual buffaloes, demonstrating that the highest level of discrimination was achieved by these typing methods. One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates from buffaloes. A novel M. bovisspoligotype (SB1474), which is reported for the first time in this study, was observed in 14.3%of isolates from buffaloes. Based on the observed genetic relationships, the findings suggest independent introductions from at least three unrelated sources. These findings improve the knowledge regarding the diversity of circulating M. bovis strains in the HiP. © 2011. The Authors.
Authors & Co-Authors
Hlokwe, Tiny Motlatso
South Africa, Onderstepoort
Arc-onderstepoort Veterinary Research
Jenkins, Akinbowale O.
South Africa, Pretoria
University of Pretoria
Streicher, Elizabeth Maria
South Africa, Stellenbosch
Stellenbosch University
Venter, Estelle Hildegard
South Africa, Pretoria
University of Pretoria
Cooper, David V.
South Africa, Cascade
Ezemvelo Kzn Wildlife
Godfroid, Jacques
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Michel, Anita Luise
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 35
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.4102/ojvr.v78i1.232
ISSN:
00302465
e-ISSN:
22190635
Research Areas
Genetics And Genomics
Study Locations
South Africa