Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Differential preservation of endogenous human and microbial DNA in dental calculus and dentin
Scientific Reports, Volume 8, No. 1, Article 9822, Year 2018
Notification
URL copied to clipboard!
Description
Dental calculus (calcified dental plaque) is prevalent in archaeological skeletal collections and is a rich source of oral microbiome and host-derived ancient biomolecules. Recently, it has been proposed that dental calculus may provide a more robust environment for DNA preservation than other skeletal remains, but this has not been systematically tested. In this study, shotgun-sequenced data from paired dental calculus and dentin samples from 48 globally distributed individuals are compared using a metagenomic approach. Overall, we find DNA from dental calculus is consistently more abundant and less contaminated than DNA from dentin. The majority of DNA in dental calculus is microbial and originates from the oral microbiome; however, a small but consistent proportion of DNA (mean 0.08 ± 0.08%, range 0.007-0.47%) derives from the host genome. Host DNA content within dentin is variable (mean 13.70 ± 18.62%, range 0.003-70.14%), and for a subset of dentin samples (15.21%), oral bacteria contribute > 20% of total DNA. Human DNA in dental calculus is highly fragmented, and is consistently shorter than both microbial DNA in dental calculus and human DNA in paired dentin samples. Finally, we find that microbial DNA fragmentation patterns are associated with guanine-cytosine (GC) content, but not aspects of cellular structure. © 2018 The Author(s).
Authors & Co-Authors
Mann, Allison E.
Germany, Jena
Max-planck-institut Für Menschheitsgeschichte
United States, Norman
The University of Oklahoma
Ziesemer, Kirsten A.
Netherlands, Leiden
Universiteit Leiden
Schroeder, Hannes
Netherlands, Leiden
Universiteit Leiden
Denmark, Copenhagen
Københavns Universitet
Ozga, Andrew T.
United States, Norman
The University of Oklahoma
United States, Tempe
Arizona State University
Sankaranarayanan, Krithivasan
United States, Norman
The University of Oklahoma
Hofman, Courtney A.
United States, Norman
The University of Oklahoma
Fellows Yates, James A.
Germany, Jena
Max-planck-institut Für Menschheitsgeschichte
Salazar-García, Domingo C.
Germany, Jena
Max-planck-institut Für Menschheitsgeschichte
Spain, Bilbao
Ikerbasque, Basque Foundation for Science
Frohlich, Bruno
United States, Hanover
Dartmouth College
United States, Washington, D.c.
Smithsonian Institution
Aldenderfer, Mark Steven
United States, Merced
Uc Merced
Hoogland, Menno L.P.
Netherlands, Leiden
Universiteit Leiden
Stone, Anne C.
United States, Tempe
Arizona State University
Lewis, Cecil M.
United States, Norman
The University of Oklahoma
Krause, Johannes
Germany, Jena
Max-planck-institut Für Menschheitsgeschichte
Hofman, Corinne L.
Netherlands, Leiden
Universiteit Leiden
Bos, Kirsten I.
Germany, Jena
Max-planck-institut Für Menschheitsgeschichte
Warinner, Christina G.
Germany, Jena
Max-planck-institut Für Menschheitsgeschichte
United States, Norman
The University of Oklahoma
Statistics
Citations: 66
Authors: 17
Affiliations: 11
Identifiers
Doi:
10.1038/s41598-018-28091-9
ISSN:
20452322
Research Areas
Genetics And Genomics
Health System And Policy