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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Density interactions among streptococcus pneumoniae, haemophilus influenzae and staphylococcus aureus in the nasopharynx of young Peruvian children
Pediatric Infectious Disease Journal, Volume 32, No. 1, Year 2013
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Description
Streptococcus pneumoniae, Haemophilus influenzae and Staphylococcus aureus are commonly carried in the nasopharynx of young children, and have been speculated to interact with each other. Although earlier studies used cultures alone to assess these interactions, the addition of real-time quantitative polymerase chain reaction (qPCR) provides further insight into these interactions. We compared results of culture and qPCR for the detection of these 3 bacteria in 446 nasopharynx samples collected from 360 healthy young children in a prospective cohort study in the Peruvian Andes. Patterns of concurrent bacterial colonization were studied using repeated measures logistic regression models with generalized estimating equations. Spearman correlation coefficients were used to assess correlations among bacterial densities. At a bacterial density <10 colony forming units/mL measured by qPCR, culture detected significantly less carriers (P < 0.0001) for all 3 pathogens, than at a bacterial density >10 colony forming units/mL. In addition, there was a positive association between S. pneumoniae and H. influenzae colonization measured by both culture (odds ratio [OR] 3.11-3.17, P < 0.001) and qPCR (OR 1.95-1.97, P < 0.01). The densities of S. pneumoniae and H. influenzae, measured by qPCR, were positively correlated (correlation coefficient 0.32, P < 0.001). A negative association was found between the presence of S. pneumoniae and Staphylococcus aureus in carriage with both culture (OR 0.45, P = 0.024) and qPCR (OR 0.61, P < 0.05). The impact of density on detection by culture and the observed density-related interactions support use of qPCR in additional studies to examine vaccine effects on diverse bacterial species. © 2012 Lippincott Williams & Wilkins.
Authors & Co-Authors
Chien, Yuwen
United States, Atlanta
Emory University
Vidal, Jorge E.
United States, Atlanta
Emory University
Grijalva, Carlos Gabriel
Unknown Affiliation
Bozio, Catherine H.
United States, Atlanta
Emory University
Edwards, Kathryn M.
Unknown Affiliation
Williams, John V.
United States, Nashville
Vanderbilt University School of Medicine
Verástegui, Héctor A.
Peru, Lima
Instituto de Investigacion Nutricional
Hartinger, Stella Maria
Switzerland, Basel
Universitat Basel
Lanata, Claudio Franco
Peru, Lima
Instituto de Investigacion Nutricional
Klugman, K. P.
United States, Atlanta
Emory University
Statistics
Citations: 80
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1097/INF.0b013e318270d850
ISSN:
15320987
Research Areas
Maternal And Child Health
Study Design
Cohort Study
Case-Control Study
Study Approach
Quantitative