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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
SXT-related integrating conjugative element and IncC plasmids in Vibrio cholerae O1 strains in Eastern Africa
Journal of Antimicrobial Chemotherapy, Volume 63, No. 3, Year 2009
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Description
Objectives: The objective of this study was to investigate the extent of resistance patterns and associated mobile genetic elements in epidemic V. cholerae O1 El Tor strains isolated from Eastern Africa in the late 1990s. Methods: Self-transmissible genetic elements and associated clusters of genes encoding resistance were detected by conjugation experiments. Detection of SXT-related integrating conjugative elements (ICEs) and associated antibiotic resistance genes was performed by PCR to amplify the SXT element-integrase gene (int), right SXT element-chromosome junction (attP-prfC) and genes conferring resistance to chloramphenicol (floR), sulfamethoxazole (sulIl), streptomycin (strA) and trimethoprim (dfrA1). Genomic relatedness was established by random amplified polymorphic DNA patterns. Results: Of 224 strains analysed, 200 isolates exhibited resistance to four or more antimicrobials. An IncC plasmid, encoding resistance to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole and trimethoprim, conferred multidrug resistance to 113 strains isolated from Somalia and Ethiopia, whereas an SXT-related ICE, encoding resistance to chloramphenicol, streptomycin, sulfamethoxazole and trimethoprim, conferred multidrug resistance to 74 strains isolated from Sudan, Kenya and Tanzania. Conclusions: This study has shown the spreadof SXT-related ICEs among V. cholerae O1 African isolates. It has also highlighted the role of two distinct genetic elements in conferring multiple resistance to the two distinct groups of V. cholerae O1 strains that, in the late 1990s, spread through Eastern Africa, a critical geographic region for the persistence and transmission of cholera to the entire continent. © The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Authors & Co-Authors
Pugliese, N.
Italy, Bari
Università Degli Studi Di Bari Aldo Moro
Maimone, Francesco
Italy, Rome
Sapienza Università Di Roma
Scrascia, Maria
Italy, Bari
Università Degli Studi Di Bari Aldo Moro
Materu, Sadiki F.
Kenya, Nairobi
Amref Health Africa
Pazzani, C.
Italy, Bari
Università Degli Studi Di Bari Aldo Moro
Statistics
Citations: 37
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1093/jac/dkn542
ISSN:
03057453
e-ISSN:
14602091
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Locations
Ethiopia
Kenya
Somalia
Sudan
Tanzania