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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Identification of novel PAMP-triggered phosphorylation and dephosphorylation events in arabidopsis thaliana by quantitative phosphoproteomic analysis
Journal of Proteome Research, Volume 13, No. 4, Year 2014
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Description
Signaling cascades rely strongly on protein kinase-mediated substrate phosphorylation. Currently a major challenge in signal transduction research is to obtain high confidence substrate phosphorylation sites and assign them to specific kinases. In response to bacterial flagellin, a pathogen-associated molecular pattern (PAMP), we searched for rapidly phosphorylated proteins in Arabidopsis thaliana by combining multistage activation (MSA) and electron transfer dissociation (ETD) fragmentation modes, which generate complementary spectra and identify phosphopeptide sites with increased reliability. Of a total of 825 phosphopeptides, we identified 58 to be differentially phosphorylated. These peptides harbor kinase motifs of mitogen-activated protein kinases (MAPKs) and calcium-dependent protein kinases (CDPKs), as well as yet unknown protein kinases. Importantly, 12 of the phosphopeptides show reduced phosphorylation upon flagellin treatment. Since protein abundance levels did not change, these results indicate that flagellin induces not only various protein kinases but also protein phosphatases, even though a scenario of inhibited kinase activity may also be possible. © 2014 American Chemical Society.
Authors & Co-Authors
Rayapuram, Naganand
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Evry
Université D'evry Val D'essonne
Bonhomme, Ludovic
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Evry
Université D'evry Val D'essonne
France, Clermont-ferrand
Génétique Diversité et Ecophysiologie Des Céréales Gdec
Bigeard, Jean
France, Evry
Université D'evry Val D'essonne
Haddadou, Kahina
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Evry
Université D'evry Val D'essonne
Przybylski, Cédric
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Evry
Université D'evry Val D'essonne
Hirt, Heribert
France, Evry
Université D'evry Val D'essonne
Saudi Arabia, Thuwal
King Abdullah University of Science and Technology
Pflieger, Delphine
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Evry
Université D'evry Val D'essonne
Statistics
Citations: 40
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1021/pr401268v
ISSN:
15353893
e-ISSN:
15353907
Study Approach
Quantitative