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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Molecular analysis of erythrocyte invasion in Plasmodium falciparum isolates from Senegal
Infection and Immunity, Volume 75, No. 7, Year 2007
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Description
The human malaria parasite, Plasmodium falciparum, utilizes multiple ligand-receptor interactions for the invasion of human erythrocytes. Members of the reticulocyte binding protein homolog (PfRh) family have been shown to be critical for directing parasites to alternative erythrocyte receptors that define invasion pathways. Recent studies have identified gene amplification, sequence polymorphism, and variant expression of PfRh paralogs as mechanisms underlying discrimination between pathways for invasion. In this study, we find considerable heterogeneity in the invasion profiles of clonal, uncultured P. falciparum parasite isolates from a low-transmission area in Senegal. Molecular analyses revealed minimal variation in protein expression levels of the PfRh ligands, PfRh1, PfRh2a, and PfRh2b, and an absence of gene amplification in these isolates. However, significant sequence polymorphism was found within repeat regions of PfRh1, PfRh2a, and PfRh2b. Furthermore, we identified a large sequence deletion (∼0.58 kb) in the C-terminal region of the PtRh2b gene at a high prevalence in this population. In contrast to findings of earlier studies, we found no associations between specific sequence variants and distinct invasion pathways. Overall these data highlight the importance of region-specific elaborations in PfRh sequence and expression polymorphisms, which has important implications in our understanding of how the malaria parasite responds to polymorphisms in erythrocyte receptors and/or evades the immune system. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC1932937/bin/iai_75_7_3531__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC1932937/bin/iai_75_7_3531__supplemental_figureC__Converted_.zip
Authors & Co-Authors
Jennings, Cameron V.
United States, Boston
Harvard T.h. Chan School of Public Health
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Ahouidi, Ambroise Dioum
United States, Boston
Harvard T.h. Chan School of Public Health
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Zilversmit, Martine
United States, Cambridge
Harvard University
Bei, Amy Kristine
United States, Boston
Harvard T.h. Chan School of Public Health
Rayner, Julian C.
United States, Birmingham
The University of Alabama at Birmingham
Sarr, Ousmane
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Ndir, Oumar
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Wirth, Dyann F.
United States, Boston
Harvard T.h. Chan School of Public Health
Mboup, Souleymane
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Duraisingh, Manoj Theodore
United States, Boston
Harvard T.h. Chan School of Public Health
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Statistics
Citations: 48
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1128/IAI.00122-07
ISSN:
00199567
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Senegal