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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium
Food Microbiology, Volume 27, No. 7, Year 2010
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Description
The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 °C and 37 °C, reaching a maximum production of 1.0 × 109 AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity. © 2010 Elsevier Ltd.
Authors & Co-Authors
Todorov, S. D.
Brazil, Sao Paulo
Universidade de São Paulo
France, Nantes
Oniris - Ecole Nationale Vétérinaire, Agroalimentaire et de L’alimentation
South Africa, Stellenbosch
Stellenbosch University
Wachsman, Mónica Beatriz
Argentina, Buenos Aires
Universidad de Buenos Aires
Tomé, Elisabetta
Venezuela, Caracas
Universidad Central de Venezuela
Dousset, Xavier
France, Nantes
Oniris - Ecole Nationale Vétérinaire, Agroalimentaire et de L’alimentation
Destro, Maria Teresa
Brazil, Sao Paulo
Universidade de São Paulo
Dicks, Leon Milner Theodore
South Africa, Stellenbosch
Stellenbosch University
Franco, B. D.G.M.
Brazil, Sao Paulo
Universidade de São Paulo
Vaz-Velho, Manuela
Portugal, Viana do Castelo
Escola Superior de Tecnologia e Gestão
Drider, Djamel
France, Nantes
Oniris - Ecole Nationale Vétérinaire, Agroalimentaire et de L’alimentation
Statistics
Citations: 165
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1016/j.fm.2010.05.001
e-ISSN:
10959998
Research Areas
Food Security
Genetics And Genomics