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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Using poly-N-acetyl glucosamine gel matrix to deliver IL-12 with anti-schistosomasis vaccination
Journal of Infection in Developing Countries, Volume 4, No. 5, Year 2010
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Description
Background: Interleukin (IL)-12 is a potential adjuvant in a variety of diseases including schistosomiasis. The clinical use of IL-12, however, is limited by the toxicity associated with its systemic administration. We have developed a novel delivery system (designated F2 gel matrix) composed of poly-N-acetyl glucosamine that has the dual properties of sustaining the release of proteins (e.g. interleukins) and adjuvant effects. The main aim of this study was to use a mouse model to test whether IL-12 released from F2 gel can induce adjuvant effects in the schistosomiasis setting as compared to those obtained after systemic delivery of IL-12. Methodology: First, we compared the toxicity induced by paracrine (delivered by F2 gel) and systemic IL-12. Second, we compared the induction of cytokines induced by paracrine and systemic IL-12. Third, we compared the adjuvant effects of paracrine and systemic IL-12-based prophylactic vaccination against schistosomiasis using soluble worm antigen preparation (SWAP). Results: IL-12 released from F2 gel did not induce significant toxicity measured by alanine aminotransferase (ALT). We found similar serum levels of IFN-γ, TNF-α and IL-2 after paracrine and systemic IL-12 treatments. We also found that vaccination with F2 gel/SWAP/IL-12 induced higher anti-schistosomal effects than IL-12/SWAP as evidenced by 1) the decrease in the total liver egg counts; 2) the reduction in the granuloma size and fibrotic reaction in the liver; and 3) the amelioration of the liver functions. Conclusion: Collectively, these results indicate that IL-12-F2 gel delivery approach could be considered as a potential strategy for the treatment of schistosomiasis. © 2010 Salem et al.
Authors & Co-Authors
Salem, Mohamed Labib
United States, Charleston
Medical University of South Carolina
Egypt, Tanta
Faculty of Science
United States, Boston
Marine Polymer Technology Inc.
Attia, W. Y.
United States, Charleston
Medical University of South Carolina
El-Sayed El-Bolkiny, Y.
Egypt, Tanta
Faculty of Science
Al-Sharkawi, Ismail Moustafa
Egypt, Tanta
Faculty of Science
Demcheva, Marina V.
Unknown Affiliation
Vournakis, John N.
Unknown Affiliation
Statistics
Citations: 8
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.3855/jidc.541
Research Areas
Infectious Diseases