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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Mycoplasma gallisepticum strain differentiation by arbitrary primer pcr (rapd) fingerprinting
Molecular and Cellular Probes, Volume 8, No. 4, Year 1994
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Description
We demonstrate here that the arbitrary primer polymerase-chain-reaction-based DNA fingerprinting method (also termed random amplified polymorphic DNA or RAPD) can be used to distinguish among strains of the avian pathogen Mycoplasma gallisepticum. Ten base oligonucleotide primers were used individually to prime DNA synthesis from genomic DNAs. Strain-specific arrays of DNA fragments were generated, which allowed us to identify and group isolates. Isolates of M. synoviae, M. gallinarum and M. iners yielded arrays of DNA fragments that differed markedly from those generated from the M. gallisepticum isolates using the same arbitrary primers. These results show that the RAPD fingerprinting method distinguishes genetically different strains of M. gallisepticum and indicates that it should be valuable for monitoring transmission of this pathogen. © 1994 Academic Press Limited.
Authors & Co-Authors
Geary, Steven J.
United States, Cairo
University of Connecticut
Forsyth, Mark H.
United States, Cairo
University of Connecticut
Saoud, S. Aboul
United States, Cairo
University of Connecticut
Egypt, Cairo
Veterinary Serum and Vaccine Research Institute Vsvri
Wang, G.
United States, Storrs
University of Connecticut
Berg, Douglas E.
United States, St. Louis
Washington University School of Medicine in St. Louis
Berg, C. M.
United States, Storrs
University of Connecticut
Statistics
Citations: 6
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1006/mcpr.1994.1042
ISSN:
08908508
Research Areas
Genetics And Genomics