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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Global Renal Gene Expression Profiling Analysis in B
2
-Kinin Receptor Null Mice: Impact of Diabetes
PLoS ONE, Volume 7, No. 9, Article e44714, Year 2012
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Description
Diabetic nephropathy (DN), the leading cause of end-stage renal failure, is clinically manifested by albuminuria and a progressive decline in glomerular filtration rate. The risk factors and mechanisms that contribute to the development and progression of DN are still incompletely defined. To address the involvement of bradykinin B2-receptors (B2R) in DN, we used a genome wide approach to study the effects of diabetes on differential renal gene expression profile in wild type and B2R knockout (B2R-/-) mice. Diabetes was induced with streptozotocin and plasma glucose levels and albumin excretion rate (AER) were measured at predetermined times throughout the 23 week study period. Longitudinal analysis of AER indicated that diabetic B2R-/-D null mice had a significantly decreased AER levels compared to wild type B2R+/+D mice (P = 0.0005). Results from the global microarray study comparing gene expression profiles among four groups of mice respectively: (B2R+/+C, B2R+/+D, B2R-/-C and B2R-/-D) highlighted the role of several altered pathological pathways in response to disruption of B2R and to the diabetic state that included: endothelial injury, oxidative stress, insulin and lipid metabolism and inflammatory process with a marked alteration in the pro-apoptotic genes. The findings of the present study provide a global genomics view of biomarkers that highlight the mechanisms and putative pathways involved in DN. © 2012 Jaffa et al.
Authors & Co-Authors
Jaffa, Miran A.
Lebanon, Beirut
American University of Beirut
Kobeissy, Firas H.
Lebanon, Beirut
American University of Beirut
Hariri, Moustafa Al
Lebanon, Beirut
American University of Beirut
Chalhoub, Hussein
Lebanon, Beirut
American University of Beirut
Eid, Assaad A.
Lebanon, Beirut
American University of Beirut
Ziyadeh, Fuad N.
Lebanon, Beirut
American University of Beirut
Jaffa, Ayad A.
Lebanon, Beirut
American University of Beirut
United States, Charleston
Medical University of South Carolina
Statistics
Citations: 19
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1371/journal.pone.0044714
e-ISSN:
19326203
Research Areas
Cancer
Genetics And Genomics
Noncommunicable Diseases
Violence And Injury
Study Design
Cohort Study