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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
The Mtb proteome library: A resource of assays to quantify the complete proteome of mycobacterium tuberculosis
Cell Host and Microbe, Volume 13, No. 5, Year 2013
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Description
Research advancing our understanding of Mycobacterium tuberculosis (Mtb) biology and complex host-Mtb interactions requires consistent and precise quantitative measurements of Mtb proteins. We describe the generation and validation of a compendium of assays to quantify 97% of the 4,012 annotated Mtb proteins by the targeted mass spectrometric method selected reaction monitoring (SRM). Furthermore, we estimate the absolute abundance for 55% of all Mtb proteins, revealing a dynamic range within the Mtb proteome of over four orders of magnitude, and identify previously unannotated proteins. As an example of the assay library utility, we monitored the entire Mtb dormancy survival regulon (DosR), which is linked to anaerobic survival and Mtb persistence, and show its dynamic protein-level regulation during hypoxia. In conclusion, we present a publicly available research resource that supports the sensitive, precise, and reproducible quantification of virtually any Mtb protein by a robust and widely accessible mass spectrometric method. © 2013 Elsevier Inc.
Authors & Co-Authors
Schubert, Olga T.
Switzerland, Zurich
Eth Zürich
Switzerland, Zurich
Systems Biology Graduate School
Mouritsen, Jeppe
Switzerland, Zurich
Eth Zürich
Switzerland, Zurich
Molecular Life Science (mls) Graduate School
Ludwig, Christina
Switzerland, Zurich
Eth Zürich
Röst, Hannes L.
Switzerland, Zurich
Eth Zürich
Switzerland, Zurich
Systems Biology Graduate School
Rosenberger, George
Switzerland, Zurich
Eth Zürich
Switzerland, Zurich
Systems Biology Graduate School
Arthur, Patrick K.
Ghana, Accra
University of Ghana
Claassen, Manfred
Switzerland, Zurich
Eth Zürich
Campbell, David S.
United States, Seattle
Institute for Systems Biology
Sun, Zhi
United States, Seattle
Institute for Systems Biology
Farrah, Terry
United States, Seattle
Institute for Systems Biology
Gengenbacher, Martin
Germany, Berlin
Max Planck Institute for Infection Biology
Maiolica, Alessio
Switzerland, Zurich
Eth Zürich
Kaufmann, Stefan Hugo Ernst
Germany, Berlin
Max Planck Institute for Infection Biology
Moritz, Robert L.
United States, Seattle
Institute for Systems Biology
Aebersold, Ruedi H.
Switzerland, Zurich
Eth Zürich
Switzerland, Zurich
Universität Zürich
Statistics
Citations: 166
Authors: 15
Affiliations: 7
Identifiers
Doi:
10.1016/j.chom.2013.04.008
ISSN:
19313128
e-ISSN:
19346069
Study Approach
Quantitative