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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Novel giant siphovirus from Bacillus anthracis features unusual genome characteristics
PLoS ONE, Volume 9, No. 1, Article e85972, Year 2014
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Description
Here we present vB-BanS-Tsamsa, a novel temperate phage isolated from Bacillus anthracis, the agent responsible for anthrax infections in wildlife, livestock and humans. Tsamsa phage is a giant siphovirus (order Caudovirales), featuring a long, flexible and non-contractile tail of 440 nm (not including baseplate structure) and an isometric head of 82 nm in diameter. We induced Tsamsa phage in samples from two different carcass sites in Etosha National Park, Namibia. The Tsamsa phage genome is the largest sequenced Bacillus siphovirus, containing 168,876 bp and 272 ORFs. The genome features an integrase/recombinase enzyme, indicative of a temperate lifestyle. Among bacterial strains tested, the phage infected only certain members of the Bacillus cereus sensu lato group (B. anthracis, B. cereus and B. thuringiensis) and exhibited moderate specificity for B. anthracis. Tsamsa lysed seven out of 25 B. cereus strains, two out of five B. thuringiensis strains and six out of seven B. anthracis strains tested. It did not lyse B. anthracis PAK-1, an atypical strain that is also resistant to both gamma phage and cherry phage. The Tsamsa endolysin features a broader lytic spectrum than the phage host range, indicating possible use of the enzyme in Bacillus biocontrol. © 2014 Ganz et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3903500/bin/pone.0085972.s001.docx
Authors & Co-Authors
Ganz, Holly H.
Unknown Affiliation
Law, Christina
Unknown Affiliation
Schmuki, Martina
Unknown Affiliation
Eichenseher, Fritz
Unknown Affiliation
Calendar, Richard
Unknown Affiliation
Loessner, Martin J.
Unknown Affiliation
Getz, Wayne Marcus
Unknown Affiliation
Korlach, Jonas
Unknown Affiliation
Beyer, Wolfgang
Unknown Affiliation
Klumpp, Jochen
Unknown Affiliation
Statistics
Citations: 24
Authors: 10
Affiliations: 8
Identifiers
Doi:
10.1371/journal.pone.0085972
e-ISSN:
19326203
Study Locations
Namibia