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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Shift to Pseudomonic Acid B Production in P. fluorescens NCIMB10586 by Mutation of Mupirocin Tailoring Genes mupO, mupU, mupV, and macpE
Chemistry and Biology, Volume 12, No. 7, Year 2005
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Description
Mupirocin, a polyketide-derived antibiotic from Pseudomonas fluorescens NCIMB10586, is a mixture of pseudomonic acids (PA) that target isoleucyl-tRNA synthase. The mup gene cluster encodes both type I polyketide synthases and monofunctional enzymes that should play a role during the conversion of the product of the polyketide synthase into the active antibiotic (tailoring). By in-frame deletion analysis of selected tailoring open-reading frames we show that mupQ, mupS, mupT, and mupW are essential for mupirocin production, whereas mupO, mupU, mupV, and macpE are essential for production of PA-A but not PA-B. Therefore, PA-B is not simply produced by hydroxylation of PA-A but is either a precursor of PA-A or a shunt product. In the mupW mutant, a new metabolite lacking the tetrahydropyran ring is produced, implicating mupW in oxidation of the 16-methyl group. ©2005 Elsevier Ltd All rights reserved.
Authors & Co-Authors
Cooper, Sian M.
United Kingdom, Birmingham
University of Birmingham
United Kingdom, Cambridge
Biotica Technology Ltd
Laosripaiboon, Wanpen
United Kingdom, Bristol
University of Bristol
Rahman, Ayesha Sabah
United Kingdom, Birmingham
University of Birmingham
Hothersall, Joanne
United Kingdom, Birmingham
University of Birmingham
El-Sayed, Ahmed K.A.
United Kingdom, Birmingham
University of Birmingham
Egypt, Mansoura
Mansoura University
Winfield, Christopher J.
United Kingdom, Bristol
University of Bristol
Crosby, John C.
United Kingdom, Bristol
University of Bristol
Cox, Russell John
United Kingdom, Bristol
University of Bristol
Simpson, Thomas J.
United Kingdom, Bristol
University of Bristol
Thomas, Christopher M.
United Kingdom, Birmingham
University of Birmingham
Statistics
Citations: 27
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/j.chembiol.2005.05.015
ISSN:
10745521
Research Areas
Cancer
Genetics And Genomics