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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
NMR metabolomics of thrips (frankliniella occidentalis) resistance in senecio hybrids
Journal of Chemical Ecology, Volume 35, No. 2, Year 2009
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Description
Western flower thrips (Frankliniella occidentalis) has become a key insect pest of agricultural and horticultural crops worldwide. Little is known about host plant resistance to thrips. In this study, we investigated thrips resistance in F2 hybrids of Senecio jacobaea and Senecio aquaticus. We identified thrips-resistant hybrids applying three different bioassays. Subsequently, we compared the metabolomic profiles of these hybrids applying nuclear magnetic resonance spectroscopy (NMR). The new developments of NMR facilitate a wide range coverage of the metabolome. This makes NMR especially suitable if there is no a priori knowledge of the compounds related to herbivore resistance and allows a holistic approach analyzing different chemical compounds simultaneously. We show that the metabolomes of thrips-resistant and -susceptible hybrids differed considerably. Thrips-resistant hybrids contained higher amounts of the pyrrolizidine alkaloids (PA), jacobine, and jaconine, especially in younger leaves. Also, a flavanoid, kaempferol glucoside, accumulated in the resistant plants. Both PAs and kaempferol are known for their inhibitory effect on herbivores. In resistant and susceptible F2 hybrids, young leaves showed less thrips damage than old leaves. Consistent with the optimal plant defense theory, young leaves contained increased levels of primary metabolites such as sucrose, raffinose, and stachyose, but also accumulated jacaranone as a secondary plant defense compound. Our results prove NMR as a promising tool to identify different metabolites involved in herbivore resistance. It constitutes a significant advance in the study of plant-insect relationships, providing key information on the implementation of herbivore resistance breeding strategies in plants. © The Author(s) 2009.
Authors & Co-Authors
Leiss, Kirsten A.
Netherlands, Leiden
Institute of Biology Leiden
Choi, Y. H.
Netherlands, Leiden
Institute of Biology Leiden
Abdel-Farid, Ibrahim Bayoumi
Netherlands, Leiden
Institute of Biology Leiden
Egypt, Aswan
Faculty of Science
Verpoorte, Rob
Netherlands, Leiden
Institute of Biology Leiden
Klinkhamer, Peter G.L.
Netherlands, Leiden
Institute of Biology Leiden
Statistics
Citations: 185
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1007/s10886-008-9586-0
ISSN:
00980331
e-ISSN:
15731561
Research Areas
Cancer
Health System And Policy