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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Adenovirus-mediated overexpression of human tissue plasminogen activator prevents peritoneal adhesion formation/reformation in rats
Surgery, Volume 146, No. 1, Year 2009
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Description
Background: Tissue-plasminogen activator (tPA) demonstrated beneficial effects on peritoneal adhesion formation; however, its short half-life limits its continual fibrinolytic effect. Therefore, we delivered adenovirus encoding tPA to prevent adhesions. Methods: Rats were subjected to peritoneal injury and assigned to two protocols. In de novo adhesion protocol, adenovirus encoding human tPA gene (Ad-htPA) was instilled after peritoneal injury in group1 (n = 22), whereas group 2 received phosphate-buffered saline (PBS) (n = 24). In recurrent adhesion protocol, group 1 (n = 15) received the same Ad-htPA dose after adhesiolysis and group 2 (n = 13) received PBS. Adhesion severity was scored 1 week after ad-htPA instillation. Adhesions were analyzed for htPA mRNA by reverse transcription-polymerase chain reaction and levels of htPA, and fibrinolytic inhibitors PAI-1, TIMP-1, and TGF-β1 were measured using enzyme-linked immunosorbent assay. Results: htPA mRNA and protein were only expressed in adhesions from treated groups. A reduction in adhesion scores (P < .01) and in fibrinolytic inhibitors (P < .001) occurred in the treatment groups. Also, negative correlation was found (r = -.69, P < .01) between adhesion scores and htPA protein, but a positive correlation was found (r = .90, P < .01) between adhesion score and fibrinolytic inhibitors. No bleeding or wound complications were encountered. Conclusion: Administration of adenovector encoding htPA is safe and decreased de novo and recurrent peritoneal adhesions. © 2009 Mosby, Inc. All rights reserved.
Authors & Co-Authors
Atta, Hussein M.
Egypt, Minya
Faculty of Medicine
Al-Hendy, Ayman A.
Egypt, Minya
Faculty of Medicine
El-Rehany, Mahmoud Abdel Aziz
United States, Galveston
The University of Texas Medical Branch at Galveston
Dewerchin, Mieke
Belgium, Leuven
Vesalius Research Center
Abdel-Raheim, Salama R.
United States, Galveston
The University of Texas Medical Branch at Galveston
Ghany, Hend Abdel
United States, Galveston
The University of Texas Medical Branch at Galveston
Fouad, Rasha
United States, Galveston
The University of Texas Medical Branch at Galveston
Statistics
Citations: 11
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1016/j.surg.2009.02.018
Research Areas
Genetics And Genomics
Violence And Injury