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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
A comparison of the sensitivity and fecal egg counts of the McMaster egg counting and Kato-Katz thick smear methods for soil-transmitted helminths
PLoS Neglected Tropical Diseases, Volume 5, No. 6, Article e1201, Year 2011
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Description
Background: The Kato-Katz thick smear (Kato-Katz) is the diagnostic method recommended for monitoring large-scale treatment programs implemented for the control of soil-transmitted helminths (STH) in public health, yet it is difficult to standardize. A promising alternative is the McMaster egg counting method (McMaster), commonly used in veterinary parasitology, but rarely so for the detection of STH in human stool. Methodology/Principal Findings: The Kato-Katz and McMaster methods were compared for the detection of STH in 1,543 subjects resident in five countries across Africa, Asia and South America. The consistency of the performance of both methods in different trials, the validity of the fixed multiplication factor employed in the Kato-Katz method and the accuracy of these methods for estimating 'true' drug efficacies were assessed. The Kato-Katz method detected significantly more Ascaris lumbricoides infections (88.1% vs. 75.6%, p<0.001), whereas the difference in sensitivity between the two methods was non-significant for hookworm (78.3% vs. 72.4%) and Trichuris trichiura (82.6% vs. 80.3%). The sensitivity of the methods varied significantly across trials and magnitude of fecal egg counts (FEC). Quantitative comparison revealed a significant correlation (Rs >0.32) in FEC between both methods, and indicated no significant difference in FEC, except for A. lumbricoides, where the Kato-Katz resulted in significantly higher FEC (14,197 eggs per gram of stool (EPG) vs. 5,982 EPG). For the Kato-Katz, the fixed multiplication factor resulted in significantly higher FEC than the multiplication factor adjusted for mass of feces examined for A. lumbricoides (16,538 EPG vs. 15,396 EPG) and T. trichiura (1,490 EPG vs. 1,363 EPG), but not for hookworm. The McMaster provided more accurate efficacy results (absolute difference to 'true' drug efficacy: 1.7% vs. 4.5%). Conclusions/Significance: The McMaster is an alternative method for monitoring large-scale treatment programs. It is a robust (accurate multiplication factor) and accurate (reliable efficacy results) method, which can be easily standardized. © 2011 Levecke et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3114752/bin/pntd.0001201.s001.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC3114752/bin/pntd.0001201.s002.xls
Authors & Co-Authors
Levecke, Bruno
Belgium, Ghent
Universiteit Gent
Behnke, Jerzy Marian B.
United Kingdom, Nottingham
University of Nottingham
Ajjampur, Sitara Swarna Rao
India, Vellore
Christian Medical College, Vellore
Albonico, Marco
Tanzania, Mkokotoni, Zanzibar
Pemba Public Health Laboratory
Ame, Shaali Makame
Tanzania, Mkokotoni, Zanzibar
Pemba Public Health Laboratory
Charlier, Johannes
Belgium, Ghent
Universiteit Gent
Geiger, Stefan Michael
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Hoa, Nguyen Thi Viet
Viet Nam, Hanoi
National Institute for Malariology
Ngassam, Romuald Isaka Kamwa
Cameroon, Yaounde
Université de Yaoundé I
Kotze, Andrew C.
Australia, Canberra
Commonwealth Scientific and Industrial Research Organisation
McCarthy, James S.
Australia, Brisbane
The University of Queensland
Montresor, Antonio
Switzerland, Geneva
Organisation Mondiale de la Santé
Periago, Maria Victoria
Brazil, Rio de Janeiro
Fundacao Oswaldo Cruz
Roy, Sheela
India, Vellore
Christian Medical College, Vellore
Tchuem Tchuenté, Louis Albert
Cameroon, Yaounde
Université de Yaoundé I
Thach, Dang Thi Cam
Viet Nam, Hanoi
National Institute for Malariology
Vercruysse, Jozef
Belgium, Ghent
Universiteit Gent
Statistics
Citations: 161
Authors: 17
Affiliations: 10
Identifiers
Doi:
10.1371/journal.pntd.0001201
ISSN:
19352727
e-ISSN:
19352735
Study Approach
Quantitative