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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Optimization of extraction conditions of total phenolics, antioxidant activities, and anthocyanin of oregano, thyme, terebinth, and pomegranate
Journal of Food Science, Volume 75, No. 7, Year 2010
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Description
The purpose of this study was to evaluate the total phenolic extracts and antioxidant activity and anthocyanins of varieties of the investigated plants. These plants include oregano, thyme, terebinth, and pomegranate. The optimum extraction conditions including temperature and solvent of the extraction process itself were investigated. Total phenolic and anthocyanin extracts were examined according to Folin-Ciocalteu assay and Rabino and Mancinelli method, respectively. The effect of different extracting solvents and temperatures on extracts of phenolic compounds and anthocyanins were studied. Plant samples were evaluated for their antioxidant chemical activity by 2, 2-diphenyl-1-picrylhydrazl assay, to determine their potential as a source of natural antioxidant. Results showed that all tested plants exhibited appreciable amounts of phenolic compounds. The methanolic extract (60 °C) of sour pomegranate peel contained the highest phenolic extract (4952.4 mg/100 g of dry weight). Terebinth green seed had the lowest phenolic extract (599.4 mg/100 g of dry weight). Anthocyanins ranged between 3.5 (terebinth red seed) and 0.2 mg/100 g of dry material (thyme). Significant effect of different extracting solvents and temperatures on total phenolics and anthocyanin extracts were found. The methanol and 60 °C of extraction conditions found to be the best for extracting phenolic compounds. The distilled water and 60 °C extraction conditions found to be the best for extracting anthocyanin. © 2010 Institute of Food Technologists®.
Authors & Co-Authors
Rababah, Taha M.
Jordan, Irbid
Jordan University of Science and Technology
Banat, F. A.
Jordan, Irbid
Jordan University of Science and Technology
Rababah, Anfal
Jordan, Irbid
Jordan University of Science and Technology
Ereifej, Khalil I.
Jordan, Irbid
Jordan University of Science and Technology
Yang, Wade
United States, Gainesville
University of Florida
Statistics
Citations: 101
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1111/j.1750-3841.2010.01756.x
ISSN:
00221147
e-ISSN:
17503841
Research Areas
Environmental
Food Security