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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Adaptation of a duck influenza A virus in quail
Journal of Virology, Volume 86, No. 3, Year 2012
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Description
Quail are thought to serve as intermediate hosts of influenza A viruses between aquatic birds and terrestrial birds, such as chickens, due to their high susceptibility to aquatic-bird viruses, which then adapt to replicate efficiently in their new hosts. However, does replication of aquatic-bird influenza viruses in quail similarly result in their efficient replication in humans? Using sialic acid-galactose linkage-specific lectins, we found both avian (sialic acid-α2-3-galactose [Siaα2-3Gal] linkages on sialyloligosaccharides)- and human (Siaα2-6Gal)-type receptors on the tracheal cells of quail, consistent with previous reports. We also passaged a duck H3N2 virus in quail 19 times. Sequence analysis revealed that eight mutations accumulated in hemagglutinin (HA) during these passages. Interestingly, many of the altered HA amino acids found in the adapted virus are present in human seasonal viruses, but not in duck viruses. We also found that stepwise stalk deletion of neuraminidase occurred during passages, resulting in reduced neuraminidase function. Despite some hemagglutinin mutations near the receptor binding pocket, appreciable changes in receptor specificity were not detected. However, reverse-genetics-generated viruses that possessed the hemagglutinin and neuraminidase of the quail-passaged virus replicated significantly better than the virus possessing the parent HA and neuraminidase in normal human bronchial epithelial cells, whereas no significant difference in replication between the two viruses was observed in duck cells. Further, the quail-passaged but not the original duck virus replicated in human bronchial epithelial cells. These data indicate that quail can serve as intermediate hosts for aquatic-bird influenza viruses to be transmitted to humans. © 2012, American Society for Microbiology.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3264366/bin/supp_86_3_1411__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3264366/bin/supp_86.3.1411_TableS1.doc
Authors & Co-Authors
Yamada, Shinya
Japan, Tokyo
The University of Tokyo
Takada, Ayato
Japan, Sapporo
Hokkaido University
Ito, Toshihiro
Japan, Tottori
Faculty of Agriculture, Tottori University
Suzuki, Takashi
Japan, Shizuoka
University of Shizuoka
Suzuki, Yasuo
Japan, Kasugai
Chubu University
Japan, Shizuoka
University of Shizuoka
Kida, Hiroshi
Japan, Sapporo
Hokkaido University
Horimoto, Taisuke
Japan, Tokyo
The University of Tokyo
Rivailler, Pierre
United States, Atlanta
Centers for Disease Control and Prevention
Donis, Ruben Omar
United States, Atlanta
Centers for Disease Control and Prevention
Kawaoka, Yoshihiro K.
Japan, Tokyo
The University of Tokyo
Japan, Kobe
Kobe University
United States, Madison
University of Wisconsin-madison
Japan, Kawaguchi
Japan Science and Technology Agency
Statistics
Citations: 29
Authors: 10
Affiliations: 11
Identifiers
Doi:
10.1128/JVI.06100-11
ISSN:
10985514
Research Areas
Genetics And Genomics
Violence And Injury