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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Detection of Yersinia pestis using real-time PCR in patients with suspected bubonic plague
Molecular and Cellular Probes, Volume 25, No. 1, Year 2011
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Description
Yersinia (Y.) pestis, the causative agent of plague, is endemic in natural foci of Asia, Africa, and America. Real-time PCR assays have been described as rapid diagnostic tools, but so far none has been validated for its clinical use. In a retrospective clinical study we evaluated three real-time PCR assays in two different assay formats, 5'-nuclease and hybridization probes assays. Lymph node aspirates from 149 patients from Madagascar with the clinical diagnosis of bubonic plague were investigated for the detection of Y. pestis DNA. Results of real-time PCR assays targeting the virulence plasmids pPCP1 (pla gene), and pMT1 (caf1, Ymt genes) were compared with an F1-antigen immunochromatographic test (ICT) and cultivation of the organism. Out of the 149 samples an infection with Y. pestis was confirmed by culture in 47 patients while ICT was positive in 88 including all culture proven cases. The best real-time PCR assay was the 5'-nuclease assay targeting pla which was positive in 120 cases. In conclusion, the 5'-nuclease assay targeting pla can be recommended as diagnostic tool for establishing a presumptive diagnosis when bubonic plague is clinically suspected. © 2010 Elsevier Ltd.
Authors & Co-Authors
Riehm, Julia M.
Germany, Munich
Institut Für Mikrobiologie Der Bundeswehr
Rahalison, Lila
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Scholz, Holger Christian
Germany, Munich
Institut Für Mikrobiologie Der Bundeswehr
Pfeffer, Martin
Unknown Affiliation
Al Dahouk, Sascha
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Germany, Berlin
Bundesinstitut Für Risikobewertung
Neubauer, Heinrich K.J.
Germany, Tubingen
Friedrich-loeffler-institute
Tomaso, Herbert
Germany, Tubingen
Friedrich-loeffler-institute
Statistics
Citations: 38
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1016/j.mcp.2010.09.002
ISSN:
08908508
Research Areas
Genetics And Genomics
Study Design
Cohort Study
Study Locations
Madagascar