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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
IgA antibodies against the Epstein-Barr nuclear antigen1 as a valuable biomarker for the diagnosis of nasopharyngeal carcinoma in Tunisian patients
Journal of Medical Virology, Volume 81, No. 8, Year 2009
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Description
Serological tests for Epstein-Barr virus (EBV) have been used for many years as diagnostic predictors of nasopharyngeal carcinoma. It has been shown previously that the conventional immunofluorescence assay has a limited diagnostic value, especially in young patients from North African area. In the search for more reliable immunoglobulin (Ig) G or IgA antibody markers for the diagnosis of nasopharyngeal carcinoma, immunoblot analysis was performed using a full spectrum of EBV proteins. Sera were collected from 108 patients with nasopharyngeal carcinoma and three control groups composed of 18 patients with lymphoma, 18 other patients with autoimmune diseases and 55 healthy EBV carriers. It was observed that the IgA Epstein-Barr nuclear antigen 1 (EBNA1), IgA early antigen (EA)-p138 and IgG EA-p138 antibodies represent the most specific anti-EBV responses in either young or older patients with nasopharyngeal carcinoma which yield higher positive rates compared to the three control groups. Since the IgA EBNA1 response showed the highest sensitivity value for the detection of nasopharyngeal carcinoma, a novel enzyme-linked immunosorbent assay (ELISA) was established using a GST-EBNA1 protein expressed in bacteria, containing the P-threonine EBNA1 subtype cloned from DNA EBV sequence of C15 xenograft cells. Detection rates were 85.7% and 94.9% in young and older patients with nasopharyngeal carcinoma respectively, while only 3.6%, 11.1%, and 16.6% in healthy EBV carriers, patients with lymphoma and patients with autoimmune diseases, respectively. Thus, IgA EBNA1 ELISA may be useful for early diagnosis and mass screening of nasopharyngeal carcinoma in Tunisia even in young patients. © 2009 Wiley-Liss, Inc.
Authors & Co-Authors
Ayadi, Wajdi
Tunisia, Sfa
Hôpital Habib Bour-guiba
Tunisia, Sfax
Faculty of Medicine of Sfax
Karray-Hakim, Héla
Tunisia, Sfa
Hôpital Habib Bour-guiba
Feki, Lamia
Tunisia, Sfa
Hôpital Habib Bour-guiba
Khabir, Abdelmajid
Tunisia, Sfa
Hôpital Habib Bour-guiba
Sellami-Boudawara, Tahiya
Tunisia, Sfa
Hôpital Habib Bour-guiba
Ghorbel, Abdelmonem
Tunisia, Sfa
Hôpital Habib Bour-guiba
Daoud, Jamel L.
Tunisia, Sfa
Hôpital Habib Bour-guiba
Frikha, Mounir F.
Tunisia, Sfa
Hôpital Habib Bour-guiba
Hammami, Adenène
Tunisia, Sfa
Hôpital Habib Bour-guiba
Statistics
Citations: 15
Authors: 9
Affiliations: 2
Identifiers
Doi:
10.1002/jmv.21532
ISSN:
01466615
e-ISSN:
10969071
Research Areas
Cancer
Genetics And Genomics
Study Locations
Tunisia