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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Purification and modes of antifungal action by vicia faba cv. Egypt trypsin inhibitor
Journal of Agricultural and Food Chemistry, Volume 58, No. 19, Year 2010
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Description
A new 15 kDa Bowman-Birk type trypsin inhibitor (termed VFTI-E1) from fava beans (Vicia faba cv. Egypt 1) was isolated using liquid chromatography. Though it exhibited substantial homology in N-terminal amino acid sequence to other protease inhibitors, VFTI-E1 showed antiproteolytic activity against trypsin (Ki 11.9 × 10-9 M) but hardly any activity against chymotrypsin. It demonstrated antifungal activity toward the filamentous fungus Valsa mali with an IC50 of 20 μM. The mechanism of its antifungal action toward V. mali included (1) induction of alteration of hyphal morphology, (2) growth inhibition by chitin deposition at hyphal tips, and (3) permeabilization of fungal membrane. The antifungal activity of VFTI-E1 was dependent on the ambient ionic strength as increasing concentrations of NaCl, CaCl2, and MgCl2 diminished the activity. The membranolytic action of VFTI-E1 was confined to fungus, but not exerted on human and rabbit erythrocytes. This study sheds light on the mode of hyphal growth inhibitory activity of protease inhibitors with antifungal activity. The antifungal activity of VFTI-E1 amplifies the scope of its potential applications. © 2010 American Chemical Society.
Authors & Co-Authors
Fang, Evandro Fei
Hong Kong, Hong Kong
Chinese University of Hong Kong, Faculty of Medicine
Hassanin, Abdallah A.
Egypt, Zagazig
Faculty of Agriculture
Wong, Jack Ho
Hong Kong, Hong Kong
Chinese University of Hong Kong, Faculty of Medicine
Bah, Clara Shui Fern
New Zealand, Dunedin
University of Otago
Soliman, Said Saad
Egypt, Zagazig
Faculty of Agriculture
Ng, Tzibun
Hong Kong, Hong Kong
Chinese University of Hong Kong, Faculty of Medicine
Statistics
Citations: 32
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1021/jf102277k
ISSN:
00218561
e-ISSN:
15205118
Study Locations
Egypt
Mali