Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Molecular prospecting for European Diplostomum (Digenea: Diplostomidae) reveals cryptic diversity
International Journal for Parasitology, Volume 43, No. 1, Year 2013
Notification
URL copied to clipboard!
Description
We believe this study is the first attempt to address molecular prospecting for species diversity of Diplostomum (Digenea: Diplostomidae) in Europe. A database linking sequences from the barcode region of the cytochrome c oxidase subunit 1 (cox1) mitochondrial gene and from the internal transcribed spacer cluster (ITS1-5.8S-ITS2) of the rRNA gene was generated for larval and adult parasites of snails, fish and gulls from central Europe. Analyses of the novel cox1 dataset revealed the presence of six genetically distinct Diplostomum lineages in the snail and fish populations studied in the River Ruhr drainage (Germany). ITS1-5.8S-ITS2 sequences from a representative subset of isolates supported the delineation detected by cox1. Molecular elucidation of the life-cycles of Diplostomum spathaceum and Diplostomum pseudospathaceum in central Europe was achieved by matching multiple sequences for isolates from natural infections in snails, fish and birds identified on the basis of the morphology of all life-cycle stages. Comparative analyses restricted to the ITS1 rDNA region and incorporating sequences for six European and seven North American Diplostomum spp. retrieved from GenBank, corroborated the results of the molecular prospecting based on the cox1 dataset. Taken together, these analyses depicted 20 molecularly characterised species and lineages of Diplostomum including three complexes of genetically distinct lineages i.e. '. Diplostomum mergi', '. Diplostomum baeri' and '. Diplostomum huronense', that require further appraisal with the application of molecular, morphological and experimental approaches. Two of the species and 10 of the lineages (arguably species) delineated in the datasets studied originate from central and northern Europe thus indicating a substantial unrecognized genetic diversity inferred from molecular evidence on Diplostomum spp. in Europe. © 2012 Australian Society for Parasitology Inc.
Authors & Co-Authors
Georgieva, Simona
Czech Republic, Ceske Budejovice
Biology Centre of Academy of Sciences of the Czech Republic
Czech Republic, Ceske Budejovice
Jihočeská Univerzita V Českých Budějovicích
Soldánová, Miroslava
Czech Republic, Ceske Budejovice
Biology Centre of Academy of Sciences of the Czech Republic
Germany, Duisburg
Universität Duisburg-essen
Pérez-Del-Olmo, Ana
Germany, Duisburg
Universität Duisburg-essen
Spain, Cerdanyola Del Valles
Universitat Autònoma de Barcelona
Sitko, Jiljí
Unknown Affiliation
Sures, Bernd
Germany, Duisburg
Universität Duisburg-essen
Kostadinova, Aneta
Czech Republic, Ceske Budejovice
Biology Centre of Academy of Sciences of the Czech Republic
Bulgaria, Sofia
The Bulgarian Academy of Sciences
Statistics
Citations: 98
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1016/j.ijpara.2012.10.019
ISSN:
18790135
Research Areas
Genetics And Genomics
Study Design
Case-Control Study