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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Transcriptomic responses to heat stress and nickel in the mussel Mytilus galloprovincialis
Aquatic Toxicology, Volume 148, Year 2014
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Description
The exposure of marine organisms to stressing agents may affect the level and pattern of gene expression. Although many studies have examined the ecological effects of heat stress on mussels, little is known about the physiological mechanisms that maybe affected by co-exposure to heat stress and environmental contaminants such as nickel (Ni). In the present work, we investigated the effects of simultaneous changes in temperature and Ni supply on lysosomal membrane stability (LMS) and malondialdehyde accumulation (MDA) in the digestive gland (DG) of the blue mussel Mytilus galloprovincialis (Lam.). To elucidate how the molecular response to environmental stressors is modulated, we employed a cDNA microarray with 1673 sequences to measure relative transcript abundances in the DG of mussels exposed to Ni along with a temperature increase. A two-way ANOVA revealed that temperature and Ni rendered additive effects on LMS and MDA accumulation, increasing the toxic effects of metal cations. Ni loads in the DG were also affected by co-exposure to 26. °C. In animals exposed only to heat stress, functional genomics analysis of the microarray data (171differentially expressed genes (DEGs)) highlighted seven biological processes, largely dominated by the up-regulation of folding protein-related genes and the down-regulation of genes involved in cell migration and cellular component assembly. Exposure to Ni at 18. °C and 26. °C yielded 188 and 262 DEGs, respectively, exhibiting distinct patterns in terms of biological processes. In particular, the response of mussels exposed to Ni at 26. °C was characterized by the up-regulation of proteolysis, ribosome biogenesis, response to unfolded proteins, and catabolic-related genes, as well as the down-regulation of genes encoding cellular metabolic processes. Our data provide new insights into the transcriptomic response in mussels experiencing temperature increases and Ni exposure; these data should be carefully considered in view of the biological effects of heat stress, particularly in polluted areas. © 2014 Elsevier B.V.
Authors & Co-Authors
Banni, Mohamed
Italy, Vercelli
Università Degli Studi Del Piemonte Orientale Amedeo Avogadro
Tunisia, Sousse
Isa
Hajer, Attig
Tunisia, Sousse
Isa
Sforzini, Susanna
Italy, Vercelli
Università Degli Studi Del Piemonte Orientale Amedeo Avogadro
Oliveri, Caterina
Italy, Vercelli
Università Degli Studi Del Piemonte Orientale Amedeo Avogadro
Flavio, Mignone
Italy, Vercelli
Università Degli Studi Del Piemonte Orientale Amedeo Avogadro
Hamadi, Boussetta
Tunisia, Sousse
Isa
Aldo, Viarengo
Italy, Vercelli
Università Degli Studi Del Piemonte Orientale Amedeo Avogadro
Statistics
Citations: 29
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.aquatox.2014.01.004
ISSN:
0166445X
e-ISSN:
18791514
Research Areas
Cancer
Environmental
Genetics And Genomics
Noncommunicable Diseases