Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
PPE and PE-PGRS proteins of Mycobacterium marinum are transported via the type VII secretion system ESX-5
Molecular Microbiology, Volume 73, No. 3, Year 2009
Notification
URL copied to clipboard!
Description
ESX-5 is one of the five type VII secretion systems found in mycobacteria. These secretion systems are also known as ESAT-6-like secretion systems. Here, we have determined the secretome of ESX-5 by a proteomic approach in two different strains of Mycobacterium marinum. Comparison of the secretion profile of wild-type strains and their ESX-5 mutants showed that a number of PE-PGRS and PPE-MPTR proteins are dependent on ESX-5 for transport. The PE and PPE protein families are unique to mycobacteria, are highly expanded in several pathogenic species, such as Mycobacterium tuberculosis and M. marinum, and certain family members are cell surface antigens associated with virulence. Using a monoclonal antibody directed against the PGRS domain we showed that nearly all PE-PGRS proteins that are recognized by this antibody are missing in the supernatant of ESX-5 mutants. In addition to PE-PGRS and PPE proteins, the ESX-5 secretion system is responsible for the secretion of a ESAT-6-like proteins. Together, these data show that ESX-5 is probably a major secretion pathway for mycobacteria and that this system is responsible for the secretion of recently evolved PE-PGRS and PPE proteins. © 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd.
Authors & Co-Authors
Abdallah, Abdallah Musa
Netherlands, Amsterdam
Amsterdam Umc - Vrije Universiteit Amsterdam
Verboom, Theo
Netherlands, Amsterdam
Amsterdam Umc - Vrije Universiteit Amsterdam
Weerdenburg, Eveline M.
Netherlands, Amsterdam
Amsterdam Umc - Vrije Universiteit Amsterdam
Gey van Pittius, Nicolaas Claudius
Netherlands, Amsterdam
Amsterdam Umc - Vrije Universiteit Amsterdam
Mahasha, Phetole Walter
Netherlands, Amsterdam
Amsterdam Umc - Vrije Universiteit Amsterdam
Jiménez, Connie R.
South Africa, Cape Town
Stellenbosch University, Faculty of Medicine and Health Sciences
Parra, Marcela
United States, Bethesda
Fda Center for Biologics Evaluation and Research
Cadieux, Nathalie
United States, Bethesda
Fda Center for Biologics Evaluation and Research
Brennan, Michael James
United States, Bethesda
Fda Center for Biologics Evaluation and Research
.Appelmelk, Ben J.
Netherlands, Amsterdam
Amsterdam Umc - Vrije Universiteit Amsterdam
Bitter, Wilbert
Netherlands, Amsterdam
Amsterdam Umc - Vrije Universiteit Amsterdam
Statistics
Citations: 276
Authors: 11
Affiliations: 3
Identifiers
Doi:
10.1111/j.1365-2958.2009.06783.x
ISSN:
0950382X
e-ISSN:
13652958