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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Probing the N-terminal β-sheet conversion in the crystal structure of the human prion protein bound to a nanobody
Journal of the American Chemical Society, Volume 136, No. 3, Year 2014
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Description
Prions are fatal neurodegenerative transmissible agents causing several incurable illnesses in humans and animals. Prion diseases are caused by the structural conversion of the cellular prion protein, PrPC, into its misfolded oligomeric form, known as prion or PrPSc. The canonical human PrPC (HuPrP) fold features an unstructured N-terminal part (residues 23-124) and a well-defined C-terminal globular domain (residues 125-231). Compelling evidence indicates that an evolutionary N-terminal conserved motif AGAAAAGA (residues 113-120) plays an important role in the conversion to PrPSc. The intrinsic flexibility of the N-terminal has hampered efforts to obtain detailed atomic information on the structural features of this palindromic region. In this study, we crystallized the full-length HuPrP in complex with a nanobody (Nb484) that inhibits prion propagation. In the complex, the prion protein is unstructured from residue 23 to 116. The palindromic motif adopts a stable and fully extended configuration to form a three-stranded antiparallel β-sheet with the β1 and β2 strands, demonstrating that the full-length HuPrPC can adopt a more elaborate β0-β1-α1-β2-α2-α3 structural organization than the canonical β1-α1-β2-α2-α3 prion-like fold. From this structure, it appears that the palindromic motif mediates β-enrichment in the PrPC monomer as one of the early events in the conversion of PrPC into PrPSc. © 2013 American Chemical Society.
Authors & Co-Authors
Abskharon, Romany N.N.
Belgium, Brussels
Vrije Universiteit Brussel
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Egypt, Cairo
National Institute of Oceanography and Fisheries
Giachin, Gabriele
Italy, Trieste
Scuola Internazionale Superiore Di Studi Avanzati
Wohlkönig, Alexandre
Belgium, Brussels
Vrije Universiteit Brussel
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Soror, Sameh Hamdy
Belgium, Brussels
Vrije Universiteit Brussel
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Egypt, Helwan
Helwan University
Pardon, Els
Belgium, Brussels
Vrije Universiteit Brussel
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Legname, Giuseppe
Italy, Trieste
Scuola Internazionale Superiore Di Studi Avanzati
Steyaert, Jan
Belgium, Brussels
Vrije Universiteit Brussel
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Statistics
Citations: 94
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1021/ja407527p
ISSN:
00027863
e-ISSN:
15205126