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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
In vivo transcriptome of plasmodium falciparum reveals overexpression of transcripts that encode surface proteins
Journal of Infectious Diseases, Volume 191, No. 7, Year 2005
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Description
Infections with the human parasite Plasmodium falciparum continue to present a great challenge to global health, Fundamental questions regarding the molecular basis of virulence and immune evasion in P. falciparum have been only partially answered. Because of the parasite's intracellular location and complex life cycle, standard genetic approaches to the study of the pathogenesis of malaria have been limited. The present study presents a novel approach to the identification of the biological processes involved in host-pathogen interactions, one that is based on the analysis of in vivo P. falciparum transcripts. We demonstrate that a sufficient quantity of P. falciparum RNA transcripts can be derived from a small blood sample from infected patients for whole-genome microarray analysis. Overall, excellent correlation was observed between the transcriptomes derived from in vivo samples and in vitro samples with ring-stage P. falciparum 3D7 reference strain. However, gene families that encode surface proteins are overexpressed in vivo. Moreover, this analysis has identified a new family of hypothetical genes that may encode surface variant antigens. Comparative studies of the transcriptomes derived from in vivo samples and in vitro 3D7 samples may identify important strategies used by the pathogen for survival in the human host and highlight, for vaccine development, new candidate antigens that were not previously identified through the use of in vitro cultures. © 2005 by the Infectious Diseases Society of America. All rights reserved.
Authors & Co-Authors
Daily, Johanna Patricia
United States, Boston
Harvard T.h. Chan School of Public Health
United States, Boston
Brigham and Women's Hospital
Kimura, Tomonori G.
United States, San Diego
Scripps Research Institute
Sarr, Ousmane
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Ndiaye, Oaouda
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Lukens, Amanda K.
United States, Boston
Harvard T.h. Chan School of Public Health
Zhou, Yingyao
United States, San Diego
The Genomics Institute of the Novartis Research Foundation
Ndir, Oumar
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Mboup, Souleymane
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Sultan, Ali A.
United States, Boston
Harvard T.h. Chan School of Public Health
Winzeler, Elizabeth Ann
United States, San Diego
The Genomics Institute of the Novartis Research Foundation
United States, San Diego
Scripps Research Institute
Wirth, Dyann F.
United States, Boston
Harvard T.h. Chan School of Public Health
Statistics
Citations: 112
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1086/428289
ISSN:
00221899
Research Areas
Genetics And Genomics
Infectious Diseases