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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Fine-needle aspiration, an efficient sampling technique for bacteriological diagnosis of nonulcerative Buruli ulcer
Journal of Clinical Microbiology, Volume 47, No. 6, Year 2009
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Description
Invasive punch or incisional skin biopsy specimens are currently employed for the bacteriological confirmation of the clinical diagnosis of Buruli ulcer (BU), a cutaneous infectious disease caused by Mycobacterium ulcerans. The efficacy of fine-needle aspirates (FNA) using fine-gauge needles (23G by 25 mm) for the laboratory confirmation of BU was compared with that of skin tissue fragments obtained in parallel by excision or punch biopsy. In three BU treatment centers in Benin, both types of diagnostic material were obtained from 33 clinically suspected cases of BU and subjected to the same laboratory analyses: i.e., direct smear examination, IS2404 PCR, and in vitro culture. Twenty-three patients, demonstrating 17 ulcerative and 6 nonulcerative lesions, were positive by at least two tests and were therefore confirmed to have active BU. A total of 68 aspirates and 68 parallel tissue specimens were available from these confirmed patients. When comparing the sensitivities of the three confirmation tests between FNA and tissue specimens, the latter yielded more positive results, but only for PCR was this significant. When only nonulcerative BU lesions were considered, however, the sensitivities of the confirmation tests using FNA and tissue specimens were not significantly different. Our results show that the minimally invasive FNA technique offers enough sensitivity to be used for the diagnosis of BU in nonulcerative lesions. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Eddyani, Miriam
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Fraga, Alexandra Gabriel
Portugal, Braga
Escola de Medicina da Universidade do Minho
Schmitt, F. C.
Portugal, Porto
Faculdade de Medicina da Universidade do Porto Fmup
Uwizeye, Cécile
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Fissette, Kristina
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Johnson, Christian Roch
Benin
Programme National de Lutte Contre L'ulcère de Buruli
Aguiar, Julia
Benin
Centre Sanitaire et Nutritionnel Gbemoten
Sopoh, Ghislain Emmanuel
Benin
Centre de Dépistage et de Traitement de L'ulcère de Buruli
Barogui, Yves Thierry
Benin
Centre de Dépistage et de Traitement de L'ulcère de Buruli
Meyers, Wayne M.
United States, Arlington
United States Department of Defense
Pedrosa, Jorge M.
Portugal, Braga
Escola de Medicina da Universidade do Minho
Portaels, Françoise Ç.Oise
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Statistics
Citations: 52
Authors: 12
Affiliations: 7
Identifiers
Doi:
10.1128/JCM.00197-09
ISSN:
00951137
Study Locations
Benin