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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Hyperhomocysteinemia, endothelial nitric oxide synthase polymorphism, and risk of coronary artery disease
Clinical Chemistry, Volume 52, No. 1, Year 2006
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Description
Background: Hyperhomocysteinemia is an independent, graded risk factor for coronary artery disease (CAD). The G894T variant of endothelial nitric oxide synthase (eNOS) was postulated to be associated with hyperhomocysteinemia and could influence individual susceptibility to CAD. The aims of this study were to investigate (a) the relationship of the eNOS G894T polymorphism with the presence and the severity of CAD and (b) the possible relationship between hyperhomocysteinemia and the eNOS G894T variant for the risk of CAD severity in a Tunisian population. Methods: We used PCR with restriction fragment length polymorphism analysis to detect the G894T variant of the eNOS gene in 100 patients with CAD and 120 healthy controls. The severity of CAD was expressed by the number of affected vessels. Total plasma homocysteine concentrations were determined by direct chemiluminescence assay. Results: The frequencies of the eNOS GG, GT, and TT genotypes in the CAD group were significantly different from those in the control group (45%, 44%, and 11% vs 60%, 35.8% and 4.2%, respectively; P = 0.035). There was no association between the eNOS G894T genotype frequencies and the number of stenosed vessels (P = 0.149). In the CAD group, the coexistence of the 894 GT or TT genotypes and hyperhomocysteinemia led to an increased risk of CAD severity. Conclusion: The G894T polymorphism of the eNOS gene is associated with the presence of CAD, and in conjunction with hyperhomocysteinemia, increased the risk of CAD severity in a Tunisian population. © 2006 American Association for Clinical Chemistry.
Authors & Co-Authors
Kerkeni, Mohsen
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Tunisia, Monastir
Chu Fattouma-bourguiba
France, Paris
Groupe Hospitalier Paris Saint-joseph
Addad, Faouzi
Tunisia, Monastir
Chu Fattouma-bourguiba
Chauffert, Maryline
France, Paris
Groupe Hospitalier Paris Saint-joseph
Myara, Anne
France, Paris
Groupe Hospitalier Paris Saint-joseph
Farhat, Mohamed Ben
Tunisia, Monastir
Chu Fattouma-bourguiba
Miled, Abdelhédi
Tunisia, Sousse
Hopital Farhat Hached Sousse
Maaroufi, Khira
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Trivin, François
France, Paris
Groupe Hospitalier Paris Saint-joseph
Tunisia, Sousse
Hopital Farhat Hached Sousse
France, Tours
Université de Tours
Statistics
Citations: 56
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1373/clinchem.2005.057950
ISSN:
00099147
Research Areas
Genetics And Genomics
Study Design
Randomised Control Trial
Cross Sectional Study