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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A field-adapted sampling and HPLC quantification method for lumefantrine and its desbutyl metabolite in whole blood spotted on filter paper
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Volume 876, No. 2, Year 2008
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Description
A quantitative reverse-phase HPLC method with UV detection, for lumefantrine (LF) and desbutyllumefantrine (DLF) in whole blood spotted on filter paper was developed. The analytes were stabilized on filter paper by treatment of blood with phosphoric acid (1.6 mol/L). Halofantrine was used as internal standard and the analytes were extracted from filter paper using methanol. The methanolic extract was extracted with di-isopropylether after addition of acidic phosphate buffer (pH 2). Chromatographic separation was carried out on a Zorbax Eclipse XDB-phenyl column (4.6 mm × 150 mm, particle size 5 μm) at a flow rate of 1 mL/min using a mobile phase of acetonitrile-ammonium acetate buffer (0.1 M ammonium acetate and 0.01 M acetic acid, pH 6.5) (10:90). The absorbance of the compounds was monitored at 335 nm. The average extraction recovery from filter paper ranged between 45-51% for LF and 25-33% for DLF for a concentration range between 300 and 3000 nM. Inter- and intra-assay coefficients of variation for LF and DLF were ≤9.2. Limits of quantification for LF and DLF were 300 nM. The method has been applied in malaria patients. In conclusion, a simple procedure for blood sampling and quantitative measurement of lumefantrine and desbutyllumefantrine suitable for field studies in resource-limited laboratories was developed. © 2008 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Ntale, Muhammad
Uganda, Kampala
Makerere University
Ogwal-Okeng, Jasper Watson
Uganda, Kampala
Makerere University
Mahindi, Margarita
Unknown Affiliation
Gustafsson, Lars L.
Unknown Affiliation
Beck, Olof M.L.
Unknown Affiliation
Statistics
Citations: 35
Authors: 5
Affiliations: 1
Identifiers
Doi:
10.1016/j.jchromb.2008.11.002
ISSN:
15700232
Research Areas
Infectious Diseases
Study Approach
Quantitative