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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Isolation of a human anti-HIV gp41 membrane proximal region neutralizing antibody by antigen-specific single B cell sorting
PLoS ONE, Volume 6, No. 9, Article e23532, Year 2011
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Description
Broadly neutralizing antibodies are not commonly produced in HIV-1 infected individuals nor by experimental HIV-1 vaccines. When these antibodies do occur, it is important to be able to isolate and characterize them to provide clues for vaccine design. CAP206 is a South African subtype C HIV-1-infected individual previously shown to have broadly neutralizing plasma antibodies targeting the envelope gp41 distal membrane proximal external region (MPER). We have now used a fluoresceinated peptide tetramer antigen with specific cell sorting to isolate a human neutralizing monoclonal antibody (mAb) against the HIV-1 envelope gp41 MPER. The isolated recombinant mAb, CAP206-CH12, utilized a portion of the distal MPER (HXB2 amino acid residues, 673-680) and neutralized a subset of HIV-1 pseudoviruses sensitive to CAP206 plasma antibodies. Interestingly, this mAb was polyreactive and used the same germ-line variable heavy (V H1-69) and variable kappa light chain (V K3-20) gene families as the prototype broadly neutralizing anti-MPER mAb, 4E10 (residues 672-680). These data indicate that there are multiple immunogenic targets in the C-terminus of the MPER of HIV-1 gp41 envelope and suggests that gp41 neutralizing epitopes may interact with a restricted set of naive B cells during HIV-1 infection. © 2011 Morris et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3184076/bin/pone.0023532.s001.ppt
https://efashare.b-cdn.net/share/pmc/articles/PMC3184076/bin/pone.0023532.s002.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3184076/bin/pone.0023532.s003.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3184076/bin/pone.0023532.s004.pdf
Authors & Co-Authors
Morris, Lynn
United States, Durham
Duke University School of Medicine
South Africa, Johannesburg
National Institute for Communicable Diseases
Chen, Xi
United States, Durham
Duke University School of Medicine
Alam, S. Munir
United States, Durham
Duke University School of Medicine
Tomaras, Georgia D.
United States, Durham
Duke University School of Medicine
Zhang, Ruijung
United States, Durham
Duke University School of Medicine
Marshall, Dawn Jones
United States, Durham
Duke University School of Medicine
Chen, Bing
United States, Boston
Harvard Medical School
Parks, Robert J.
United States, Durham
Duke University School of Medicine
Foulger, Andrew
United States, Durham
Duke University School of Medicine
Jaeger, Frederick H.
United States, Durham
Duke University School of Medicine
Donathan, Michele
United States, Durham
Duke University School of Medicine
Bilska, Miroslawa
United States, Durham
Duke University School of Medicine
Gray, Elin Solomonovna
South Africa, Johannesburg
National Institute for Communicable Diseases
Abdool Karim, Salim S.
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
Kepler, Thomas B.
United States, Durham
Duke University School of Medicine
Whitesides, John F.
United States, Durham
Duke University School of Medicine
Montefiori, David Charles
United States, Durham
Duke University School of Medicine
Moody, Michael Anthony
United States, Durham
Duke University School of Medicine
Liao, Huaxin
United States, Durham
Duke University School of Medicine
Haynes, Barton F.
United States, Durham
Duke University School of Medicine
Statistics
Citations: 157
Authors: 20
Affiliations: 4
Identifiers
Doi:
10.1371/journal.pone.0023532
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Infectious Diseases