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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Dynamic population changes in mycobacterium tuberculosis during acquisition and fixation of drug resistance in patients
Journal of Infectious Diseases, Volume 206, No. 11, Year 2012
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Description
Background. Drug-resistant tuberculosis poses a growing challenge to global public health. However, the diversity and dynamics of the bacterial population during acquisition of drug resistance have yet to be carefully examined.Methods.Whole-genome sequencing was performed on 7 serial Mycobacterium tuberculosis (M. tuberculosis) populations from 3 patients during different stages in the development of drug resistance. The population diversity was assessed by the number and frequencies of unfixed mutations in each sample.Results.For each bacterial population, 8-41 unfixed mutations were monitored by the fraction of single-nucleotide polymorphisms at specific loci. Among them, as many as 4 to 5 resistance-conferring mutations were transiently detected in the same single sputum, but ultimately only a single type of mutant was fixed. In addition, we identified 14 potential compensatory mutations that occurred during or after the emergence of resistance-conferring mutations.Conclusions.M. tuberculosis population within patients exhibited considerable genetic diversity, which underwent selections for most fit resistant mutant. These findings have important implications and emphasize the need for early diagnosis of tuberculosis to decrease the chance of evolving highly fit drug-resistant strains. © The Author 2012.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3488197/bin/supp_206_11_1724__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC3488197/bin/supp_jis601_jis601supp.doc
Authors & Co-Authors
Sun, Gang
China, Shanghai
Shanghai Medical College Fudan University
Luo, Tao
China, Shanghai
Shanghai Medical College Fudan University
Yang, Chongguang
China, Shanghai
Shanghai Medical College Fudan University
Wang, Shengyue
China, Shanghai
Chinese National Human Genome Center at Shanghai
Barry, Clifton Earl
United States, Bethesda
National Institutes of Health Nih
Mei, Jian
China, Shanghai
Shanghai Municipal Center for Disease Control and Prevention
Gao, Qian
China, Shanghai
Shanghai Medical College Fudan University
Statistics
Citations: 172
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1093/infdis/jis601
ISSN:
00221899
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study