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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Mesenchymal stromal cell injection protects against oxidative stress in Escherichia coli-induced acute lung injury in mice
Cytotherapy, Volume 16, No. 6, Year 2014
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Description
Background aims: Stem cells may be a promising therapy for acute respiratory distress syndrome. Recent in vivo and in vitro studies suggested that the mesenchymal stromal cells (MSCs) have anti-oxidative stress properties. We hypothesized that intravenous injection of bone marrow-derived mesenchymal stem cells (MSCs) could attenuate Escherichia coli-induced acute lung injury (ALI) in mice by controlling the oxidative stress status. Methods: Eighty mice were randomly divided into four groups: group 1 (control group) received 25 μL of saline as a vehicle; group 2 contained E coli-induced ALI mice; group 3 included mice that received MSCs before induction of ALI; group 4 included mice that received MSCs after induction of ALI. Lung samples were isolated and assayed for oxidative stress variables and histopathologic analysis. Total anti-oxidant capacity was measured in broncho-alveolar lavage. Results: Pre- and post-injury MSC injection increased survival, reduced pulmonary edema and attenuated lung injuries in ALI mice. Histologically, MSCs exhibited a considerable degree of preservation of the pulmonary alveolar architecture. An increase of anti-oxidant enzyme activities and a decrease of myeloperoxidase activity and malondialdehyde levels in the MSC recipient groups versus the ALI group were found. Furthermore, the total anti-oxidant capacity and reduced glutathione levels were significantly increased in MSCs recipient groups versus the ALI group. Weak +ve inducible nitric oxide synthase immuno-expression in groups that received MSCs was detected. Pre-injury MSC injection showed better effects than did post-injury MSC injection. Conclusions: Systemic bone marrow-derived MSC injection was effective in modulating the oxidative stress status in E coli-induced acute lung injury in mice. © 2014 International Society for Cellular Therapy.
Authors & Co-Authors
Shalaby, Sally Mahmoud Saeed
Egypt, Zagazig
Zagazig University, Faculty of Medicine
El-Shal, Amal S.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Abd-Allah, Somia H.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Selim, Assmaa O.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Selim, Sally Ahmad
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Gouda, Zienab A.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Abd El Motteleb, D. M.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Zanfaly, Hala E.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
EL-Assar, Heba M.
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Abdelazim, Shymaa
Egypt, Zagazig
Zagazig University, Faculty of Medicine
Statistics
Citations: 53
Authors: 10
Affiliations: 1
Identifiers
Doi:
10.1016/j.jcyt.2013.12.006
ISSN:
14653249
e-ISSN:
14772566
Research Areas
Cancer
Violence And Injury
Study Design
Randomised Control Trial