Association of adiponectin gene polymorphisms 276G>T with obesity and biochemical parameters in adolescents

Objective: Several studies have reported the associations between adiponectin (ADIPOQ) gene polymorphisms with obesity and insulin resistance, but the results are inconclusive. The aim of this work was to study the relationship between adiponectin gene polymorphisms 276G>T with obesity and biochemical parameters in Egyptian adolescents. Methods: Genotyping of 276G>T single nucleotide polymorphisms of adiponectin gene was carried out by PCR-RFLP analysis in 204 adolescents (104 obese and 100 non-obese). Anthropometric and biochemical parameters were measured by standard procedures. Results: Genotypes distributions of 276G>T polymorphisms were significantly different between obese and non-obese cases. Obese adolescents had a higher distribution of GT/TT genotype compared with non obese subjects. Homozygous carriers (TT) and heterozygous carriers (GT) had higher body mass index, mid upper arm circumference, waist to hip ratio, body fat percentage, fasting glucose and triglyceride levels than GG carriers. The risk of obesity was associated with presence of TT genotype, whereas with T allele. Conclusions: Presence of the TT allele at the 276 locus of the ADIPOQ gene is associated with higher triglyceride and glucose levels. The study suggests that the ADIPOQ 276G>T polymorphism may be a useful biomarker for obesity and its related complications in adolescents.