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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Antioxidant activity and cytotoxicity effect of flavonoids isolated from athrixia phylicoides
Journal of Medicinal Plants Research, Volume 4, No. 23, Year 2010
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Description
Bioassay-guided fractionation of an ethanolic extract from aerial parts of Athrixia phylicoides using silica and sephadex column chromatography led to the isolation of three flavonoids. The compounds were identified as: 5-hydroxy-6,7,8,3',4',5'-hexamethoxyflavon-3-ol (1), 3-0-demethyldigicitrin (2), and Quecertin (3). Isolated compounds together with ethanol crude extract were tested for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-spectrophotometric assay, while cytotoxicity effect was determined using XTT colorimetric assay. The crude extract showed a concentration-dependent radical scavenging activity with EC50 value of 10.64 ± 0.08 μg/ml. Compound 3 was the most potent radical scavenger, exhibiting EC50 value of 1.27 ± 0.25 μg/ml, followed by compound 1 and 2 showing 2.74 ± 0.10 and 3.41 ± 0.09 μg/ml respectively. The crude extract showed no or little toxicity on Vero cells at lower concentrations tested exhibiting the IC50 value of 107.8 ± 0.13 μg/ml. Compound 3 showed minimal toxicity effect by exhibiting IC50 value of 81.38 ± 0.33 μg/ml as compared to compound 2 (IC50, 28.92 ± 0.12 μg/ml) and compound 1 (IC50, 27.91 ± 0.18 μg/ml). The results obtained from this study provide a clear rationale for the medicinal uses of A. phylicoides. © 2010 Academic Journals.
Authors & Co-Authors
Mavundza, Edison Johannes
South Africa, Pretoria
University of Pretoria
Tshikalange, Thilivhali Emmanuel
South Africa, Pretoria
University of Pretoria
Lall, Namrita
South Africa, Pretoria
University of Pretoria
Hussein, Ahmed Abdel Fattah
South Africa, Pretoria
University of Pretoria
Mudau, Nixwell Fhatuwani
South Africa, Sovenga
University of Limpopo
Meyer, Jacobus Johannes Marion
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 39
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.5897/jmpr10.823
ISSN:
19960875
e-ISSN:
19960875