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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A new quantitative HIV load assay based on plasma virion reverse transcriptase activity for the different types, groups and subtypes
AIDS, Volume 17, No. 3, Year 2003
Notification
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Description
Background: Plasma viral load monitoring is an integral part of the standard of care for HIV-infected patients in industrialized countries. In developing countries, viral load assay is either unaffordable or hindered by on-site maintenance and/or technical problems. Objectives: To evaluate a new and simple quantitative assay for plasma HIV reverse transcriptase (RT) activity; and to compare RT activity-based and RNA-based quantification in plasma samples from patients infected by different subtypes of HIV-1 group-M, HIV-1 group-O and HIV-2. Methods: The RT-based viral load assay involves separation of the virion-protected RT and quantification of its activity with an enzyme immunoassay. Plasma viraemia was quantified both by RT activity and by RNA copies in 322 samples from 236 HIV-1 group M-infected patients, including serial samples from 54 patients. Samples from 49 patients infected by HIV-1 group O or HIV-2 were also tested. Results: RT activity and RNA copies were detected in 70% of plasma samples; respectively 25% and 1% of samples contained detectable RNA copies or RT activity alone. Measured RT activity corresponded to 48%, 96% and 100% of samples with 1.7-4.0 log10, 4.1-4.8 log10 and 4.9-6.7 log10 RNA copies/ml, respectively. The values of the two assays correlated independently of the HIV subtype (P < 0.0001) and group/type (P < 0.03). Patient follow-up showed a similar pattern of viraemia with the two assays. Conclusion: Plasma RT activity assay is a simple, cheap and reliable alternative for HIV viral load determination. As such, it could be particularly valuable for diagnosis and treatment monitoring in developing countries. © 2003 Lippincott Williams & Wilkins.
Authors & Co-Authors
Braun, Joséphine
France, Rouen
Hopital Charles Nicolle
Plantier, Jean Christophe
France, Rouen
Hopital Charles Nicolle
Tuaillon, Édouard
France, Rouen
Hopital Charles Nicolle
Gueudin, Marie
France, Rouen
Hopital Charles Nicolle
Damond, Florence
France, Paris
Hôpital Bichat-claude-bernard Ap-hp
Malmsten, Anders
Sweden, Uppsala
Uppsala Universitet
Sweden, Uppsala
Cavidi Tech ab
Corrigan, Gary E.
Sweden, Uppsala
Cavidi Tech ab
Simon, Franćois
France, Rouen
Hopital Charles Nicolle
Statistics
Citations: 67
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1097/00002030-200302140-00006
ISSN:
02699370
Research Areas
Health System And Policy
Infectious Diseases
Study Design
Cohort Study
Study Approach
Quantitative