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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells
Molecular Cancer, Volume 6, Article 75, Year 2007
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Description
Background: The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. This study was carried out to investigate the gene expression profile of CSCs in human lung adenocarcinoma A549 cells. Results: We isolated CSCs from A549 cell line of which side population (SP) phenotype revealed several stem cell properties. After staining the cell line with Hoechst 33342 dye, the SP and non-side population (non-SP) cells were sorted using flow cytometric analysis. The mRNA expression profiles were measured using an Affymetrix GeneChip® oligonucleotide array. Among the sixty one differentially expressed genes, the twelve genes inclusive three poor prognostic genes; Aldo-keto reductase family 1, member C1/C2 (AKR1C1/C2), Transmembrane 4 L six family member 1 nuclear receptor (TM4SF1), and Nuclear receptor subfamily 0, group B, member 1 (NR0B1) were significantly up-regulated in SP compared to non-SP cells. Conclusion: This is the first report indicating the differences of gene expression pattern between SP and non-SP cells in A549 cells. We suggest that the up-regulations of the genes AKR1C1/C2, TM4SF1 and NR0B1 in SP of human adenocarcinoma A549 cells could be a target of poor prognosis in anti-cancer therapy. © 2007 Seo et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Sung, Ji-min
South Korea, Seoul
Konkuk University
Cho, Hee-jung
South Korea, Seoul
Konkuk University
Yi, Hee
South Korea, Seoul
Konkuk University
Kim, Jin-suk
South Korea, Seoul
Konkuk University
Abd El-Aty, A. M.
South Korea, Seoul
Konkuk University
Shin, Ho-chul
South Korea, Seoul
Konkuk University
Statistics
Citations: 83
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1186/1476-4598-6-75
ISSN:
14764598
Research Areas
Cancer
Genetics And Genomics
Study Design
Cross Sectional Study