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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Antimelanogenesis effect of Tunisian herb Thymelaea hirsuta extract on B16 murine melanoma cells
Experimental Dermatology, Volume 16, No. 12, Year 2007
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Description
Skin pigmentation is the result of melanogenesis that occurs in melanocytes and/or melanoma cells. Although melanogenesis is necessary for the prevention of DNA damage and cancer caused by UV irradiation, excessive accumulation of melanin can also cause melanoma. Thus, we focused on the antimelanogenesis effect of an extract from Thymelaea hirsuta, a Tunisian herb. Murine melanoma B16 cells were treated with T. hirsuta extract, and then cell viability and synthesized melanin content were measured. We found that the T. hirsuta extract decreased the synthesized melanin content in B16 cells without cytotoxicity. Tyrosinase is a key enzyme of melanogenesis and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation is known to be related to melanogenesis inhibition. To clarify its mechanism, we also determined ERK1/2 phosphorylation and tyrosinase expression level. ERK1/2 was immediately phosphorylated in cells just after treatment with the extract. The tyrosinase expression was inhibited after 24 h of stimulation with the extract. The T. hirsuta extract was fractionated, and we found that one fraction considerably decreased the melanin synthesis in B16 cells and that this fraction contains daphnanes as the main component. This indicates that our findings might be attributable to daphnanes. © 2007 The Authors Journal compilation © 2007 Blackwell Munksgaard.
Authors & Co-Authors
Kawano, Mitsuko
Japan, Tsukuba
University of Tsukuba
Matsuyama, Kyoko
Japan, Tsukuba
University of Tsukuba
Miyamae, Yusaku
Japan, Tsukuba
University of Tsukuba
Shinmoto, Hiroshi
Japan, Tsukuba
Food Research Institute, Naro
Kchouk, Mohamed Elyes
Tunisia, Hamam Lif
Centre de Biotechnologie de Borj Cédria
Morio, Takahiro
Japan, Tsukuba
University of Tsukuba
Shigemori, Hideyuki
Japan, Tsukuba
University of Tsukuba
Isoda, Hiroko
Japan, Tsukuba
University of Tsukuba
Statistics
Citations: 64
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1111/j.1600-0625.2007.00618.x
ISSN:
09066705
e-ISSN:
16000625
Research Areas
Cancer
Genetics And Genomics