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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Gut pathology and responses to the microsporidium Nosema ceranae in the honey bee Apis mellifera
PLoS ONE, Volume 7, No. 5, Article e37017, Year 2012
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Description
The microsporidium Nosema ceranae is a newly prevalent parasite of the European honey bee (Apis mellifera). Although this parasite is presently spreading across the world into its novel host, the mechanisms by it which affects the bees and how bees respond are not well understood. We therefore performed an extensive characterization of the parasite effects at the molecular level by using genetic and biochemical tools. The transcriptome modifications at the midgut level were characterized seven days post-infection with tiling microarrays. Then we tested the bee midgut response to infection by measuring activity of antioxidant and detoxification enzymes (superoxide dismutases, glutathione peroxidases, glutathione reductase, and glutathione-S-transferase). At the gene-expression level, the bee midgut responded to N. ceranae infection by an increase in oxidative stress concurrent with the generation of antioxidant enzymes, defense and protective response specifically observed in the gut of mammals and insects. However, at the enzymatic level, the protective response was not confirmed, with only glutathione-S-transferase exhibiting a higher activity in infected bees. The oxidative stress was associated with a higher transcription of sugar transporter in the gut. Finally, a dramatic effect of the microsporidia infection was the inhibition of genes involved in the homeostasis and renewal of intestinal tissues (Wnt signaling pathway), a phenomenon that was confirmed at the histological level. This tissue degeneration and prevention of gut epithelium renewal may explain early bee death. In conclusion, our integrated approach not only gives new insights into the pathological effects of N. ceranae and the bee gut response, but also demonstrate that the honey bee gut is an interesting model system for studying host defense responses. © 2012 Dussaubat et al.
Authors & Co-Authors
Dussaubat, Claudia
France, Avignon
Centre de Recherche Inrae Provence-alpes-côte D’azur
Brunet, Jean Luc
France, Avignon
Centre de Recherche Inrae Provence-alpes-côte D’azur
Colbourne, John Kenneth
United States, Bloomington
Indiana University Bloomington
Lopez, Jacqueline Ann
United States, Bloomington
Indiana University Bloomington
Martín-Hernández, Raquel R.
Unknown Affiliation
Cousin, Marianne
France, Avignon
Centre de Recherche Inrae Provence-alpes-côte D’azur
Bonnet, Marc
France, Avignon
Centre de Recherche Inrae Provence-alpes-côte D’azur
Belzunces, Luc P.
France, Avignon
Centre de Recherche Inrae Provence-alpes-côte D’azur
Moritz, Robin F.A.
Germany, Halle
Martin-luther-universität Halle-wittenberg
Le Conte, Yves
France, Avignon
Centre de Recherche Inrae Provence-alpes-côte D’azur
Alaux, Cédric
France, Avignon
Centre de Recherche Inrae Provence-alpes-côte D’azur
Statistics
Citations: 195
Authors: 11
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0037017
ISSN:
19326203
Research Areas
Genetics And Genomics