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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Role of the EphB2 receptor in autophagy, apoptosis and invasion in human breast cancer cells
Experimental Cell Research, Volume 320, No. 2, Year 2014
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Description
The Eph and Ephrin proteins, which constitute the largest family of receptor tyrosine kinases, are involved in normal tissue development and cancer progression. Here, we examined the expression and role of the B-type Eph receptor EphB2 in breast cancers. By immunohistochemistry using a progression tissue microarray of human clinical samples, we found EphB2 to be expressed in benign tissues, but strongly increased in cancers particularly in invasive and metastatic carcinomas. Subsequently, we found evidence that EphB2, whose expression varies in established cell breast lines, possesses multiple functions. First, the use of a DOX-inducible system to restore EphB2 function to low expressers resulted in decreased tumor growth in vitro and in vivo, while its siRNA-mediated silencing in high expressers increased growth. This function involves the onset of apoptotic death paralleled by caspases 3 and 9 activation. Second, EphB2 was also found to induce autophagy, as assessed by immunofluorescence and/or immunoblotting examination of the LC3, ATG5 and ATG12 markers. Third, EphB2 also has a pro-invasive function in breast cancer cells that involves the regulation of MMP2 and MMP9 metalloproteases and can be blocked by treatment with respective neutralizing antibodies. Furthermore, EphB2-induced invasion is kinase-dependent and is impeded in cells expressing a kinase-dead mutant EphB2. In summary, we identified a mechanism involving a triple role for EphB2 in breast cancer progression, whereby it regulates apoptosis, autophagy, and invasion. © 2013 Elsevier Inc.
Authors & Co-Authors
Chukkapalli, Sahiti
United States, Detroit
Wayne State University School of Medicine
Amessou, Mohamed
United States, Detroit
Wayne State University School of Medicine
Dilly, Ashok Kumar
United States, Detroit
Wayne State University School of Medicine
Dekhil, Hafedh
Saudi Arabia, Riyadh
King Saud University
Zhao, Jing
Canada, Montreal
Centre du Cancer Segal
Liu, Qiang
Canada, Montreal
Centre du Cancer Segal
Bejna, Alex
United States, Detroit
Wayne State University School of Medicine
Thomas, Ron Dan
United States, Detroit
Wayne State University School of Medicine
Bandyopadhyay, Sudeshna
United States, Detroit
Wayne State University School of Medicine
Bismar, Tarek A.
Canada, Calgary
Cumming School of Medicine
Neill, Daniel
United States, Detroit
Wayne State University School of Medicine
Azoulay, Laurent Y.
Canada, Montreal
Université Mcgill
Batist, Gerald
Canada, Montreal
Centre du Cancer Segal
Canada, Montreal
Université Mcgill
Kandouz, Mustapha
United States, Detroit
Wayne State University School of Medicine
United States, Detroit
Wayne State University
Statistics
Citations: 47
Authors: 14
Affiliations: 6
Identifiers
Doi:
10.1016/j.yexcr.2013.10.022
ISSN:
00144827
e-ISSN:
10902422
Research Areas
Cancer