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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Fibroblasts facilitate the engraftment of embryonic stem cell-derived cardiomyocytes on three-dimensional collagen matrices and aggregation in hanging drops
Stem Cells and Development, Volume 19, No. 10, Year 2010
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Description
There is growing interest in the use of cardiomyocytes purified from embryonic stem (ES) cells for tissue engineering and cardiomyoplasty. However, most transplanted cells are lost shortly after transplantation due to the lack of integration into the host tissue and subsequent apoptosis. Here we examine whether murine embryonic fibroblasts (MEFs) can support the integration of purified murine ES cell-derived cardiomyocytes in a 3-dimensional tissue culture model based on a freezed-dryed collagen matrix with tubular structure. Collagen matrix was seeded either with cardiomyocytes alone or in combination with MEFs. The collagen sponges that were transplanted with cardiomyocytes alone showed neither morphological nor functional integration of viable cells. Cardiomyocytes also did not appear to be capable of attaching quantitatively to any of 16 different 2-dimensional biomaterials. However, cardiomyocytes co-cultured with MEFs formed fiber-like structures of rod-shaped cells with organized sarcomeric structure that contracted spontaneously. Electrical coupling between cardiomyocytes was suggested by strong expression of connexin 43. In addition, MEFs as well as cardiac fibroblasts supported re-aggregation of dissociated cardiomyocytes in hanging drops in the absence of collagen matrix. We conclude that fibroblasts promote cardiomyocyte engraftment and formation of functional 3-dimensional tissue in vitro. Elucidation of the mechanism of this phenomenon may help improve the integration of cardiomyocytes in vivo. © 2010, Mary Ann Liebert, Inc.
Authors & Co-Authors
Pfannkuche, Kurt
Germany, Koln
Universität zu Köln
Neuss, Sabine
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Pillekamp, Frank
Germany, Koln
Universität zu Köln
Germany, Dusseldorf
Heinrich-heine-universität Düsseldorf
Frenzel, Lukas P.
Germany, Koln
Universität zu Köln
Attia, Wael A.
Egypt, Giza
Cairo University
Hannes, Tobias
Germany, Koln
Universität zu Köln
Salber, Jochen
Germany, Bochum
Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil Ggmbh
Hoss, Mareike
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Zenke, Martin W.
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Fleischmann, Bernd Kurt
Germany, Bonn
Universität Bonn
Hescheler, Jürgen
Germany, Koln
Universität zu Köln
Saric, Tomo
Germany, Koln
Universität zu Köln
Statistics
Citations: 34
Authors: 12
Affiliations: 6
Identifiers
Doi:
10.1089/scd.2009.0255
ISSN:
15473287
Research Areas
Cancer
Noncommunicable Diseases