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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Oxidative stress and endothelial dysfunction in aortas of aged spontaneously hypertensive rats by NOX1/2 is reversed by NADPH oxidase inhibition
Hypertension, Volume 56, No. 3, Year 2010
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Description
Arterial hypertension is associated with increased levels of reactive oxygen species, which may scavenge endothelium-derived NO and thereby diminish its vasorelaxant effects. However, the quantitatively relevant source of reactive oxygen species is unclear. Thus, this potential pathomechanism is not yet pharmacologically targetable. Several enzymatic sources of reactive oxygen species have been suggested: uncoupled endothelial NO synthase, xanthine oxidase, and NADPH oxidases. Here we show that increased reactive oxygen species formation in aortas of 12-to 14-month-old spontaneously hypertensive rats versus age-matched Wistar Kyoto rats is inhibited by the specific NADPH oxidase inhibitor VAS2870 but neither by the xanthine oxidase inhibitor oxypurinol nor the NO synthase inhibitor NG-nitro-L-arginine methyl ester. NADPH oxidase activity, as well as protein expression of its catalytic subunits, NOX1 and NOX2, was increased in the aortas of spontaneously hypertensive rats, whereas the expression of NOX4 protein, the most abundant NOX isoform, was not significantly changed. Impaired acetylcholine-induced relaxation of spontaneously hypertensive rat aortas was significantly improved by VAS2870. In conclusion, NOX1 and NOX2 but not NOX4 proteins are increased in aged spontaneously hypertensive rat aortas. Importantly, these NOX isoforms, in particular, ectopic expression of NOX1 in endothelial cells, appear to affect vascular function in an NADPH oxidase inhibitor-reversible manner. NADPH oxidases may, thus, be a novel target for the treatment of systemic hypertension. © 2010 American Heart Association, Inc.
Authors & Co-Authors
Wind, Sven
Germany, Giessen
Universität Gießen Fachbereich Medizin
Beuerlein, Knut
Germany, Giessen
Universität Gießen Fachbereich Medizin
Armitage, Melanie E.
Australia, Clayton
Monash University
Australia, Melbourne
The Florey
Taye, Ashraf Mohamed
Germany, Giessen
Universität Gießen Fachbereich Medizin
Egypt, Minya
Faculty of Pharmacy
Kumar, Arun H.S.
Germany, Giessen
Universität Gießen Fachbereich Medizin
Australia, Clayton
Monash University
Janowitz, Daniel
Germany, Giessen
Universität Gießen Fachbereich Medizin
Neff, Christina
Australia, Clayton
Monash University
Shah, Ajay M.
United Kingdom, London
King's College London
Wingler, Kirstin
Australia, Clayton
Monash University
Australia, Melbourne
The Florey
Netherlands, Maastricht
Universiteit Maastricht
Schmidt, Harald H.H.W.
Australia, Clayton
Monash University
Australia, Melbourne
The Florey
Netherlands, Maastricht
Universiteit Maastricht
Statistics
Citations: 163
Authors: 10
Affiliations: 6
Identifiers
Doi:
10.1161/HYPERTENSIONAHA.109.149187
ISSN:
0194911X
Research Areas
Noncommunicable Diseases